The Drosophila GAGA factor (GAF) has an extraordinarily diverse set of functions that include the activation and silencing of gene expression, nucleosome organization and remodeling, higher order chromosome architecture and mitosis. One hypothesis that could account for these diverse activities is that GAF is able to interact with partners that have specific and dedicated functions. To test this possibility we used affinity purification coupled with high throughput mass spectrometry to identify GAF associated partners. Consistent with this hypothesis the GAF interacting network includes a large collection of factors and complexes that have been implicated in many different aspects of gene activity, chromosome structure and function. Moreover, we show that GAF interactions with a small subset of partners is direct; however for many others the interactions could be indirect, and depend upon intermediates that serve to diversify the functional capabilities of the GAF protein.

Background Epigenetic memory plays a critical role in the establishment and maintenance of cell identities in multicellular organisms. Polycomb and trithorax group (PcG and TrxG) proteins are responsible for epigenetic memory, and in flies, they are recruited to specialized DNA regulatory elements termed polycomb response elements (PREs). Previous transgene studies have shown that PREs can silence reporter genes outside of their normal context, often by pairing sensitive (PSS) mechanism; however, their silencing activity is non-autonomous and depends upon the surrounding chromatin context. It is not known why PRE activity depends on the local environment or what outside factors can induce silencing. Results Using an attP system in Drosophila, we find that the so-called neutral chromatin environments vary substantially in their ability to support the silencing activity of the well-characterized bxdPRE. In refractory chromosomal contexts, factors required for PcG-silencing are unable to gain access to the PRE. Silencing activity can be rescued by linking the bxdPRE to a boundary element (insulator). When placed next to the PRE, the boundaries induce an alteration in chromatin structure enabling factors critical for PcG silencing to gain access to the bxdPRE. When placed at a distance from the bxdPRE, boundaries induce PSS by bringing the bxdPREs on each homolog in close proximity. Conclusion This proof-of-concept study demonstrates that the repressing activity of PREs can be induced or enhanced by nearby boundary elements. Graphical abstract

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Deep lower crustal intraplate earthquakes are infrequent and the mechanism of their occurrence is not well understood. The Narmada-Son-lineament region in central India has experienced two such events, the 1938 Satpura earthquake and the 1997 Jabalpur earthquake, having a focal depth of more than 35 km. We have estimated elastic stresses due to the crustal density and mechanical properties heterogeneities along the Hirapur-Mandla profile passing through the Jabalpur earthquake region to analyse conditions suitable for the concentration of shear stresses in the hypocentral region of this earthquake. Elastic stresses have been computed by a finite element method for a range of material parameters. The results indicate that the shear stresses generated by the density heterogeneities alone are not able to locally enhance the stress concentration in the hypocentral region. The role of mechanical properties of various crustal layers is important in achieving this localization of stresses. Among a range of material parameters analysed, the model with a mechanically strong lower crust overlying a relatively weak sub-Moho layer is able to enhance the stress concentration in the hypocentral region, implying a weaker mantle in comparison to the lower crust for this region of central India.

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