The dynamics of the pro-oxidant/antioxidant system of rabbits was studied at the local (in exudate) and systemic (blood) levels during therapy of purulent wounds in liquid medium with the use of a programmed device and the efficiency of the proposed technological method was compared with that of classical methods for the treatment of these wounds. More rapid recovery of the indicators of the pro-oxidant/antioxidant system to physiological values in the postoperative period was observed after treatment by the proposed method (7 days vs. 10 days after standard treatment), these results attest to strengthening of the adaptation potential in laboratory animals. The intensity of free radical oxidation in the exudate decreased by 26.6% in animals treated by the technological method. These data indicated acceleration of the regenerative processes at the local level.

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The aim was to investigate prooxidant-antioxidant system in the blood and brain homogenates functional activity in rats with acute hypoxia model with different deuterium/protium (D/H) ratios in drinking diet. Studies have shown that consuming DDW (–665 ‰) within 8 weeks lead to deuterium concentration decrease in blood plasma at 317 ‰ and brain at 209 ‰ of rats, in comparison to control group, consuming natural water. DDW consumption before hypoxia modeling in rats improves antioxidant defense enzymes (catalase, superoxide dismutase, glutathione peroxidase and glutathione reductase) activity in the blood, increasing its antioxidant potential by 20 %, while free radical oxidation intensity in plasma and biomolecules peroxide modification rate in erythrocytes. Also, in brain tissues consuming DDW, there were no abnormalities in catalase, superoxide dismutase activity, and it was noted increase (by 71 %) in reduced thiol-containing compounds concentration, which reduces nerve hypoxi? cell damage risk. Neuroprotective effect presence is confirmed by higher (by 32 %) antioxidant activity markers of lyophilized brain tissues, and by free radical oxidation lower intensity (by 13 %) and biomolecules oxidative modification rate (by 16 %) in these lyophilized tissues. Thus, the advisability of using DDW neuroprotective effects in cerebral circulation disturbance in experimental and clinical practice.

The effect of a reduced deuterium (D) content in the incubation medium on the survival of cultured neurons in vitro and under glucose deprivation was studied. In addition, we studied the effect of a decrease in the deuterium content in the rat brain on oxidative processes in the nervous tissue, its antioxidant protection, and training of rats in the T-shaped maze test under hypoxic conditions. For experiments with cultures of neurons, 7–8-day cultures of cerebellar neurons were used. Determination of the rate of neuronal death in cultures was carried out using propidium iodide. Acute hypoxia with hypercapnia was simulated in rats by placing them in sealed vessels with a capacity of 1 L. The effect on oxidative processes in brain tissues was assessed by changes in the level of free radical oxidation and malondialdehyde. The effect on the antioxidant system of the brain was assessed by the activity of catalase. The study in the T-maze was carried out in accordance with the generally accepted methodology, the skill of alternating right-sided and left-sided loops on positive reinforcement was developed. This work has shown that a decrease in the deuterium content in the incubation medium to a level of −357‰ has a neuroprotective effect, increasing the survival rate of cultured neurons under glucose deprivation. When exposed to hypoxia, a preliminary decrease in the deuterium content in the rat brain to −261‰ prevents the development of oxidative stress in their nervous tissue and preserves the learning ability of animals in the T-shaped maze test at the level of the control group. A similar protective effect during the modification of the 2H/1H internal environment of the body by the consumption of DDW can potentially be used for the prevention of pathological conditions associated with the development of oxidative stress with damage to the central nervous system.

The article is devoted to the main soil treatment with a combined plow. The article has a research character, which is expressed in the fact that the analysis of methods and means of mechanization for the main tillage with the turnover of a layer, as well as excerpts from the works of leading scientists on the problem, there were shown the types of patents that provided the optimal soil treatment, their shortcomings and solutions to these problems. There were determined the main trend of development of soil-processing combined machines, improvement of constructions of arable units to improve the quality of their work. Also, the form of the disk was justified, namely, there was shown the causal relationship of the presence of cuts in the disk of the tillage tool in comparison with the solid disk. There were described the principle and process of the developed combined plow. As a result of the work done, there was obtained the design of a combined plow with additional working bodies which consist of disks installed by batteries behind each body of the plow. The conclusions set out the main results achieved so far.The mechanical influence of the plow on the soil is accompanied by resistance, which value depends on many factors. Many researchers, having determined the relationship between the physical and mechanical properties of the soil and its quality processing agents, give different forms of calculations using different formulas. They are aimed at determining the traction resistance when plowing with various soil processing tools. Also, leading scientists identify several factors that affect the quality of plowing and the energy process of tillage:-physical and mechanical (hardness, stickiness, humidity, porosity, etc.) -technical (type of soil processing unit design, condition, adjustment, etc.) -technological (way of processing, depth of plowing, speed of processing, etc.) It is also generally accepted that the traction resistance of soil cultivating unit consists of useful and harmful resistance of its working bodies. The value of useful resistances depends on the size and geometric shapes of the used plows and plow bodies, the depth of processing, as well as on the friction properties of the materials used in the manufacture of working bodies. Harmful resistances depend on the friction force of field boards on the vertical wall of the furrow, on the degree of depreciation of the blades of the working bod-