Objective: A study was designed to determine changes in the amplitude of the EMG muscular activity of the Masseter and Temporalis muscles at clench in children with a Class II, division 1 malocclusion treated with the pre-orthodontic Trainer functional appliance, for 12 months. Study Design: 36 Class II, division 1 malocclusion patients (mean age 7.6 ± 1.3 years) composed the treated group and wore the functional appliance; 22 children with a similar age and malocclusion composed the untreated controls; and, 20 children with no dental malocclusion participated as normal controls. Electromyographic (EMG) muscular activity of the Temporalis and Masseter muscles were recorded before and after treatment. Results: Subjects in the treated group reported a bilateral significant increase in the muscular electrical activity in the both tested muscles (p < 0.001). After treatment, they recorded values similar to those measured in normal controls, whereas the untreated controls remained on lower values. Conclusion: These results confirm that treatment with the pre-orthodontic Trainer functional appliance significantly increases the EMG muscular activity in the Temporalis and Masseter muscles at clench in patients with Class II, division 1 malocclusion.

A technology to create a cell-seeded fibrin-based implant matching the size and shape of bone defect is required to create an anatomical implant. The aim of the study was to develop a technology of cell-seeded fibrin gel implant creation that has the same shape and size as the bone defect at the site of implantation. Using computed tomography (CT) images, molds representing bone defects were created by 3D printing. The form was filled with fibrin glue and human dental pulp stem cells (DPSC). The viability, set of surface markers and osteogenic differentiation of DPSC grown in fibrin gel along with the clot retraction time were evaluated. In mice, an alveolar bone defect was created. The defect was filled with fibrin gel seeded with mouse DPSC. After 28 days, the bone repair was analyzed with cone beam CT and by histological examination. The proliferation rate, set of surface antigens and osteogenic potential of cells grown inside the scaffold and in 2D conditions did not differ. In mice, both cell-free and mouse DPSC-seeded implants increased the bone tissue volume and vascularization. In mice with cell-seeded gel implants, the bone remodeling process was more prominent than in animals with a cell-free implant. The technology of 3D-printed forms for molding implants can be used to prepare implants using components that are not suitable for 3D printing.

The purpose of this study was to determine the effectiveness of toothpaste Parodontax in patients undergoing orthodontic treatment. The results showed that fixed orthodontic appliances deteriorated oral hygiene, increased the viscosity of saliva and reduced mineralizing capacity of saliva (MCS). Use of Parodontax toothpaste based on sodium bicarbonate improved OHI-S, reduced the viscosity of saliva, increased MCS and normalized oral microbiota.

The rapid and dynamic development of medicine, and in particular dentistry, creates a need for doctors to constantly improve and systematize their knowledge and clinical experience. Recent advances in genetics, microbiology and other innovative industries in the last decade have updated the understanding of dental pathology. In this situation, we will talk about non-carious lesions, which, on the one hand, have become increasingly common in the practice of dentists, on the other hand, the etiological and pathogenetic factors of their development have been clarified to a large extent. This could not but affect the approaches to the treatment of this type of pathology. In this manual, we tried to systematize modern data on non-carious lesions that occur before teething (during the period of follicular tissue development), focusing on the clinical course and approaches to the treatment of pathology and the prevention of other associated dental diseases. Based on our own clinical experience, we have detailed the features of the clinical course of non-carious lesions so that they are well recognizable to physicians. Particularly interesting and useful, in our opinion, the manual will be for pediatric dentists and orthodontists, students and residents studying

Relevance. Creating three-dimensional scaffolds from biodegradable materials and seeding them with stem cells derived from the oral tissues is a promising tool for guided tissue regeneration. Pulp and periodontal stem cells have a high potential for osteogenic differentiation, which biologically determines their use in surgical bone reconstruction. The experiment shows the result of using fibrin glue seeded with pulp and periodontal stem cells on the mandible of laboratory mice. The article presents the results of computed tomography and histological examination. The data provide evidence of the influence of seeded scaffolds on bone remodeling in the area of the defect.Materials and methods. The Local Ethics Committee of the North-Western State Medical University named after I.I. Mechnikov gave permission for the practical part of the research work. The study included 29 white laboratory mice. Molars were extracted and a bone defect was formed. Pulp and periodontal stem cells were obtained and cell-seeded scaffolds were made, then they were introduced into the defect area. The animals were euthanized, maxillofacial CT scan and histology of the defect area were performed 28 days after the molar extraction.Results. The oral cavity of mice was examined, molars were extracted, and teeth were morphologically examined under anesthesia. Scaffolds were synthesized and bone defects were filled. CT scans and histology results were analyzed. The bone volume increased in the main group compared to the control group.Conclusion. The fibrin glue can be used to obtain a material with mechanical characteristics sufficient for a stable shape scaffold. The study proved that the pulp stem cells enclosed in a fibrin glue-based scaffold can maintain the ability to proliferate and osteogenically differentiate. The scaffold based on fibrin glue, which we used, affected the bone remodeling process in the area of jaw defects.