Rezistentnyye k karbapenemam shtammy Pseudomonas aeruginosa rastsenivayut v kachestve kriticheski opasnykh patogenov pervogo urovnya prioriteta. Rasshifrovka mekhanizmov formirovaniya ustoychivosti k karbapenemam yavlyayetsya aktual'noy zadachey sovremennoy meditsinskoy nauki. Tsel'yu raboty bylo opisat' raznoobraziye i zakrepleniye mutatsiy, assotsiirovannykh s formirovaniyem karbapenemrezistentnosti v protsesse adaptatsii P. aeruginosa k povyshayushchimsya kontsentratsiyam meropenema. Ob"yektami issledovaniya byli izolyaty P. aeruginosa, poluchennyye pri roste referentnogo shtamma P. aeruginosa ATCC 27853 v gradiyente vozrastayushchikh kontsentratsiy meropenema. Otsenku chuvstvitel'nosti izolyatov k meropenemu vypolnyali pri pomoshchi ye-testov (epsilometricheskiy metod) s meropenemom i pri pomoshchi metoda dilyutsii antibiotika v agare. Genomy izolyatov byli sekvenirovany na polnogenomnom sekvenatore MGISEQ-2000. Poluchennyye rezul'taty pokazali, chto formirovaniye vysokikh urovney rezistentnosti k meropenemu u P. aeruginosa v eksperimente proiskhodit v korotkiye sroki (6 sutok). Evolyutsiya rezistentnosti sopryazhena s protsessom klonirovaniya, pri kotorom proiskhodit vozniknoveniye mnozhestva klonov s razlichnymi genotipami. Osnovoy klonirovaniya yavlyayetsya mutagenez, v kotoryy vovlecheny 11 genov, vklyuchaya oprD, pbuE, nalD, nalC, spoT, mlaA, mexD, mexR, oprM, mraY, pbp3. Chast' obrazovavshikhsya klonov, nezavisimo ot urovnya ikh rezistentnosti k meropenemu, ne poluchayut progressivnogo razvitiya i vytesnyayutsya boleye uspeshnymi klonami.
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