The activity of experimental disinfectant against pathogenic bee enterobacteria is shown in the article. Since the use of antibiotics in beekeeping is prohibited in Ukraine, the main task for epizootologists is to prevent the occurrence of infectious bees diseases. The use of new disinfectants and increased veterinary measures on apiaries will contribute to the prevention of bees enterobacteriosis. It has been established that the main causative agents of bee enterobacteriosis are bacteria of the species Klebsiella Pneumoniae, Enterobacter Aerogenes. The aim of our work was to determine the effect of iodine-containing disinfectant on bee cultures of enterobacteriaceae. We found that the disinfectant under study has a significant bacteriostatic effect on Klebsiella Pneumoniae microorganisms at concentrations from 1:100 to native, and a slight bactericidal effect at concentrations from 1:10 to native state. It was also investigated that the present agent has a bacteriostatic effect on Enterobacter Aerogenes microorganisms at concentrations from native state to 1:10, but no bactericidal effect was found. It is known that an important condition for disinfectants is to maintain its activity for a long time. That`s why the next stage of the research was to determine the resistance of the disinfectant depending on the storage time of the drug. We found out that after 14 days of storage of the disinfectant its antimicrobial activity plummets, especially to microorganisms of Enterobacter Aerogenes species, where both bacteriostatic and bactericidal activity were absent. And on bacteria of the Klebsiella Pneumoniae species the drug retained a slight bacteriostatic effect and the diameter of the growth inhibition zone was 10 and 8 mm (in dilutions from native to 1:5). It was shown that the experimental disinfectant effect had both bactericidal and bacteriostatic effects, but it is insufficient for the beehives, frames and beekeeping equipment disinfection in case of enterobacteriosis on apiaries. The activity of the major disinfectant components needs to be improved and investigated. We also recommend to pay attention to the factors that determine the antimicrobial potency of iodine and selenium compounds.
The article presents laboratory diagnostics (in vitro), namely, identification of pure culture of pathogenic bacteria of Klebsiella Pneumoniae species in case of enterobacteriosis in bees in winter-spring and summer-autumn times. The purpose of the study was the biochemical typification of bacteria of the species Klebsiella Pneumoniae with humane medicine methods, that isolated in the case of dysbacteriosis of bees which have a characteristic symptomatic complex of a decrease in the strength of bee families, which leads to a decrease in the resistance of the bee family, their diarrhea, crawling, and then swarming or death of bees. Contamination of beehive frames and walls of beehive by fecation leads to sharp deterioration of the apiary's veterinary and sanitary condition and significant economic damage for beekeepers. Pure culture of pathogenic bacteria served as an object for experiment. The Family of the bacteria was established earlier – Enterobacteriaceae, and was confirmed by “Zhytomyr Regional State Laboratory of the State Service of Ukraine for Food Safety and Consumer Protection”. Laboratory diagnostics of dysbiosis in bees caused by enterobacteria was performed in such a sequence: 1. Sowing of pathological material taken from sick bees on selective nutrient media for enterobacteria and extraction of pure culture; 2. Microscopy of typical colonies; 3. Determination of bacteria genus; 4. Determination of bacteria motor activity: 5. Urease test; 6. Indole test; 7. Phenyalaalanine test; 8. Study of basic enzymatic properties of bacteria. In a series of laboratory biochemical studies of pure culture microorganisms isolated from mixed culture from diseased bees the isolated bacterial strain belongs to the Family Enterobacteriaceae, Genus Klebsiella, Species Klebsiella pneumoniae. The novelty of the application of the algorithm for determining the species of Klebsiella pneumoniae enterobacteriae allows to diagnose dysbacterioses in winter-spring and summer-autumn times clearly and economically. The isolated Klebsiella pneumoniae bacteria serve as experimental cultures for testing drugs of various directions and actions in laboratory conditions and are kept at the Department of Microbiology, Pharmacology and Epizootology, Faculty of Veterinary Medicine of Polissya National University (formerly Zhytomyr National Agroecological University). Further application of complex diagnostics of enterobacteriosis of bees, including methods of biochemical typification, will allow to expand etiological factors of bee family collapse.
The beekeeping industry provides not only honey but also other medicinal products for the human race. The health of bee colonies is supported by data on the prevalence of contagious bee diseases - monitoring investigations. At present, the system for providing such surveys is imperfect. There is a need not only to analyze diseases proposed by regional laboratories but also to diagnose other common diseases. To characterize the epizootic situation of contagious diseases of bees, we systematized and analyzed official data from the Departments of the State Consumer Service of Zhytomyr, Rivne, and Volyn regions. The article presents the data of the analysis of bee diseases investigated by regional laboratories in these regions. This study's materials were reports from regional laboratories of the State Consumer Service of Zhytomyr, Rivne, and Volyn regions. The data were statistically processed, and the results were analyzed by comparing the epizootic process development indicators. The data results indicate the prevalence of varroaatosis and nosema annually in all regions studied. In the Volyn region, there is a trend toward decreasing diagnostic tests for bee diseases compared to 2019. An annual increase in diagnostic tests has been registered in the Zhytomyr and Rivne regions of Ukraine. Occasionally, regional laboratories sometimes conduct unscheduled tests for other bacterial and viral diseases of bees. Based on planned surveys alone, it is difficult to describe the prevalence of infectious diseases in these insects. Therefore, early detection of the source of the infectious agent is advisable to ensure the well-being of bee farms. Thus a monitoring system for bee diseases of viral and bacterial origin would be promising, with the primary objective of obtaining organic bee products. Such approaches would provide a more precise and more realistic picture of the epizootic state of each region.
The search for alternative methods for treating and preventing bee dysbacteriosis is a priority for beekeeping as a branch of veterinary medicine. Lime honey, buckwheat honey, flower honey, forest honey, and acacia honey were tested to evaluate their antagonistic effect against a test culture of enterobacteria of bees of Klebsiella pneumoniae species. The study was conducted in several stages: 1. Determine the activity of honey microflora against a pure culture of enterobacteria of bees of Klebsiella pneumoniae bee pathogens; 2. The identification and isolation of Bacillus subtilis – bacteria-antagonists against Klebsiella pneumoniae bee pathogens; 3. Determine the antagonistic effect of pure culture of Bacillus subtilis against a pure culture of enterobacteria of Klebsiella pneumoniae bee pathogens. The antagonistic action of honey microorganisms and the determination of the most effective honey species were established by the diffusion method in agar wells. Staining of typical colonies from different types of honey revealed bacillary colonies of Gram-positive bacilli with endospores. Isolation of clean culture was conducted by a method of Gold. The cultural, tinctorial, and morphological signs of bacteria have been consistently determined in all investigated kinds of honey and coincided with characteristics of the Bacellaceae family. Specific belonging of bacteria-competitors was identified by biochemical typing. After determining their physiological properties in reactions and tests (activity of catalase, oxidase, urease, the ability to grow at different temperatures and to ferment carbohydrate substrate), the distinguished stamms of microorganisms from the investigated kinds of honey belong to the type of Bacillus subtilis. The repeated estimation of antagonistic action of pure cultures of Bacillus subtilis (isolated from each type of honey) against a pure culture of enterobacteria of bees of Klebsiella pneumoniae species confirmed their high activity. This type of microorganisms can represent the alternative component in probiotics at the therapy of dysbiosis of bees.
The article presents the mechanisms of development and dynamics of changes in the treatment of dogs' infectious hepatitis. According to reports from doctors of veterinary medicine in clinics of small animals of Zhytomyr, the following infectious diseases are dominated in dogs: parvovirus enteritis, viral hepatitis, parainfluenic dogs, leptospirosis, carnivorous plague, trichophytosis and food toxicoinfection. One of the most common diseases in clinical practice was infectious hepatitis, which became the object of our research. The study of various aspects of the liver function pathology in diseases of viral etiology is especially relevant because of the exclusive compensatory potentials of this organ, their clinical manifestation is often found in the stage of severe morphofunctional violations, often not subject to reverse development. The purpose of our work was to study the influence Adenovirus саnіnе -a pathogen of dogs' infectious hepatitis, on the functional state of the liver, to find out the effectiveness of the drug hepato-lik in the complex intensive care of sick dogs. The subject of the study were patients with infectious dogs' hepatitis. The main diagnostic criterion was the use of express texts, which confirmed the previous diagnosis -infectious dogs' hepatitis. For the study of the effectiveness of the used treatment of infectious hepatitis, an experimental animal was developed on the organism of the dogs -from 10 patients and control -out of 10 healthy. The applied treatment for infectious dogs' hepatitis was comprehensive, and included silence, calmness and diet therapy. In order to restore the functional status of the dogs' liver with hepatitis, the treatment regimen included 5% glucose solution with 5% solution of ascorbic acid in a dose, 40% solution of glutargin and 2% solution of riboxin for 5-10 days. Assigned vitamin preparation hepavicle and used symptomatic therapy. For the normalization of the function and regeneration of the liver after the action of adenovirus, as well as to reduce the negative effects of drugs having hepatotoxic action used hepato-lik. In order to assess the efficacy of the treatment, animals were examined clinically and blood samples were taken for the conduct of studies on the 10th and 30th day of treatment. The scheme of treatment for infectious dogs' hepatitis using hepatoprotector hepatolique has been used to improve the functional state of the liver, which was manifested in the restoration of the white-oxidative, pigmentary, anti-toxic function, improvement of detoxification function, the suppression of liver inflammation, and also contributed to the preservation and restoration of the structure of hepatocytes, normalization of ammonia levels in the dog's organism, accelerated the regeneration of the liver cells, and had little positive effect on erythrocytopoies and on the general condition of the orgnism in general.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2025 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.