The aim of this work was to identify the causative agent of community-acquired pneumonia and coinfection using PCR study of biomaterial from patients.Materials and methods. PCR testing of 268 samples from 258 patients was carried out to identify RNA/DNA of viral and bacterial pathogens of respiratory infections.Results and discussion. In 43.3 % of samples SARS-CoV-2 RNA was detected, in 4.5 % – RNA/DNA of acute respiratory viral infections pathogens, in one sample – DNA of Mycoplasma pneumoniae. Co-infection was detected only in patients of the anti-tuberculosis dispensary (SARS-CoV-2 and Mycobacterium tuberculosis). In the examined patients with pneumonia, SARS-CoV-2 RNA was significantly more often detected in biomaterial from the lower respiratory tract (52 %) than in respiratory smears (8.5 %). In the first week from the onset of the disease, 19.2 % of positive samples were found, in the second – 56.5 %.
Целью настоящей работы являлось выявление возбудителя внебольничных пневмоний и ко-инфицирования с помощью исследования методом ПЦР биоматериала от пациентов.
Проведено исследование 268 проб от 258 пациентов методом ПЦР для выявления РНК/ДНК возбудителей респираторных инфекций вирусной и бактериальной природы. В 43,3% проб выявлена РНК SARS-Cov-2, в 4,5% – РНК/ДНК возбудителей ОРВИ, в одной пробе – ДНК Mycoplasma pneumoniae. Ко-инфекция SARS-Cov-2 обнаружена только с микобактериями туберкулеза у пациентов противотуберкулезного диспансера. У обследованных больных пневмонией РНК SARS-Cov-2 существенно чаще выявлялась в биоматериале из нижних дыхательных путей (52%), чем в респираторных мазках (8,5%). В первую неделю от начала заболевания обнаружено 19,2% положительных проб, во вторую – 56,5%.
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