Aim. To study the frequency of polymorphic gene variants encoding proteins of renin-angiotensin system [AGT Thr174Met (rs 4762), AGT Met235Thr (rs699), AGTR1 A1166C (rs5186)], endothelial factors [NOS3 C786T (rs2070744)], and folate cycle enzymes [MTHFR C677T (rs1801133)] in patients with various ischemic stroke severity. Methods. 98 patients with ischemic stroke verified by magnetic resonance imaging and computed tomography scan of the brain, were examined. The severity of stroke was assessed by National Institutes of Health Stroke Scale. The allelic variants of genes were typified by polymerase chain reaction with the detection of amplification products in the «real time» mode. Results. The analysis of genetic polymorphism frequency of renin-angiotensin system did not reveal statistically significant differences in the study groups. The polymorphism of NOSC786T gene in the study was also not associated with the severity of ischemic stroke. Among the studied polymorphic variants, only the C677T polymorphism of MTHFR gene was revealed to be reliably associated with severe course of acute cerebral ischemia. Conclusion. C677T polymorphism of MTHFR gene is reliably associated with severe course of acute cerebral ischemia; carriage of 677T allele of MTHFR gene in the studied category of patients can cause an increased level of homocysteine and have an adverse effect on the course of acute cerebral ischemia.

Aim. To develop a method for predicting the development of complications in rhinosinusitis patients based on the cytokine profile study. Methods. We examined 110 patients with rhinosinusitis and 30 healthy donors (control group). The patients were divided into the group without a complicated course of rhinosinusitis (first group, n=65) and the group with a complicated course of rhinosinusitis (second group, n=45). The blood serum levels of interleukin (IL)-1, IL-4, IL-8, IL-10, IL-17, IL-18, interferon (IFN)- were determined by enzyme immunoassay. Statistical analysis of the results was performed by using Microsoft Excel 2013 and Statistica 12.0 software. Statistically significant differences between the compared groups were determined by using the MannWhitney U-test, and p 0.05 was considered significant. Multivariate analysis included correlation analysis and stepwise regression analysis. The mathematical model consistency was determined by using Fisher's F-test, and p 0.05 was considered significant. Results. Changes in cytokine profile manifested by a decrease in the level of interleukin-1 (p=0.00001), interleukin-4 (p=0.045) and an increase in the level of interleukin-18 (p=0.00001) were revealed in patients with uncomplicated course of rhinosinusitis. The complicated course of rhinosinusitis was characterized by a decrease in the level of interleukin-1 (p=0.00002), interleukin-4 (p=0.049) and an increase in the level of interleukin-8 (p=0.023), interleukin-17 (p=0.00015) and interleukin-18 (p=0.0002). Comparative analysis of the first and the second groups of patients showed an increased level of interleukin-8 (p=0.00001), interleukin-17 (p=0.0001) and reduced levels of interleukin-18 (p=0.00045) in the group of patients with complicated course of rhinosinusitis. Based on interleukin-17, interleukin-8 and interleukin-18 levels, the mathematical model for predicting the development of complications in patients with rhinosinusitis was developed. Conclusion. The complicated course of rhinosinusitis was characterized by decreased levels of interleukin-1, interleukin-4 and increased levels of interleukin-17, interleukin-8 and interleukin-18, indicating a more pronounced inflammatory process; for personalized therapy, an approach based on interleukin-17, interleukin-8 and interleukin-18 levels was developed on which the development of a complicated course in patients with rhinosinusitis can be predicted.

Адрес для переписки:Баранова Надежда Ивановна ГБОУ ДПО «Пензенский институт усовершенствования врачей» Министерства здравоохранения РФ 440060, Россия, г. Пенза, ул. Стасова, 8а. Тел./факс: 8 (8412) 43-43-57. E-mail: giuv@sura.ru Address for correspondence: Baranova Nadezhda I. Penza Institute of Postgraduate Medical Education 440060, Russian Federation, Penza, Stasova str., 8a. Phone/Fax: 7 (8412) 43-43-57. E-mail: giuv@sura иммунология, 2015. Т. 17, № 2. С. 167-172. doi: 10.15789/1563-0625-2015-2-167-172 © Баранова Н.И. и соавт., 2015 Immunologiya, 2015, Vol. 17, no. 2, pp. 167-172. doi: 10.15789/1563-0625-2015 Резюме. Проведено обследование 50 пациентов с хронической аутоиммунной крапивницей (ХАК) и 48 пациентов с хронической идиопатической крапивницей (ХИК). Изучено содержание цитоки-нов IL-4, IL-10 и IL-17A в сыворотке, в спонтанной и индуцированной продукции клетками крови, а также полиморфизм генов цитокинов IL-4 (С-589Т), IL-10 (G-1082А), . Не обна-ружено различий при изучении показателей IL-4 в зависимости от генетических вариантов изуча-емых полиморфизмов у больных ХАК и ХИК. Выявлено повышение показателей IL-10 у больных ХИК, которое не имело взаимосвязи с генотипом IL-10 (G-1082A). Повышенные показатели IL-17A у пациентов с ХАК ассоциировались с гомозиготным носительством АА по сравнению с контрольной группой и гетерозиготным носительством GA по сравнению с группой ХИК. Выявленные различия полиморфизма генов цитокинов при ХАК и ХИК свидетельствуют о различиях молекулярно-генети-ческих основ формирования изучаемых форм ХК.