effects of intermittent normobaric hypoxia on the state of the cNS and cerebral circulation in children with cerebral palsy We studied the effects of intermittent normobaric hypoxia (INH) on the processes of CNS functions and cerebral circulation recovery in children with cerebral palsy (CP). Altogether, 87 patients (from 8.5 months to 12 years) with CP were examined and received the course of treatment. Clinico-neurophysiological examination was performed before the treatment and immediately after termination of the therapeutic course. Patients were divided into two groups; age and sex distributions and clinical manifestations of CP were randomized. The comparison group was formed from 34 children who received the course of the generally accepted complex therapy (medicamental treatment, massage, Bobat-therapy, Vojta-therapy at al).. The main group included 53 patients who, in addition to the same therapy, were exposed to INH using an individual apparatus for artificial mountain air, Borey-M, made in the Scientific Medico-Engineering Center NORT (Ukrainian National Academy of Sciences, Kyiv). Children of the main group were exposed to the dosed normobaric sanogenetic level hypoxia intermittently once per day. For this purpose, we used a normobaric gas hypoxic mixture (12% O 2 + 88% N 2). Each cycle included a 15-min-long episode of breathing with the gas mixture alternated by a 5-min-long episode of breathing an ambient atmospheric air. The number of hypoxic cycles was gradually increased (from one to three). The entire course of treatment included, on average, 10 sessions. After complex therapy the stable positive effects on the motor status were observed in 94% of patients of the main group (exposed to INH) and in 74% of patients of the comparison group (unexposed to INH). EEG examination showed that positive dynamics of spectral EEG components were in 70% of patients of the main group and in 56% of children of the comparison group. Doppler examination showed that brain hemodynamics was normalized in 85% of patients of the main group and in 59% of children of the comparison group. In the course of ophthalmoscopic examination, we found that the dynamics of indices of the state of the eye fundus were expressed more clearly in children of the main group than in patients of the comparison group (in 32 and 12% of patients, respectively).

There are only few studies in which the effect of intermittent normobaric hypoxia on the liver status in animals and people with arterial hypertension has been investigated. That’s why it’s necessary to carry out these studies in animals with high blood pressure. The purpose of this work was to carry out comparative studies of the effect of dosed intermittent normobaric hypoxia on the morphofunctional state of hepatocytes of normotensive (line Wistar) and spontaneously hypertensive (line SHR) rats, age 4 months. The experimental rats were daily exposed to hypoxic gas mixture (12% oxygen in nitrogen) in intermittent mode: 15 minutes deoxygenation / 15 minutes reoxygenation for 2 hours. The duration of the experiment was 28 days. Histological, morphometric and biochemical research methods were used. The histological preparations were made by the standard methods. The slides were photographed using a digital camera on the microscope "Nikon" (Japan). The morphometric analysis was performed on digital images using the computer program "Image J". The activity of cytochrome oxidase and succinate dehydrogenase enzymes in a suspension of hepatocytes mitochondria was determined by the method of R. S. Krivchenkov. An increase was observed in the size of hepatocytes and their nucleus, in the number of binuclear hepatocytes and nucleolus in the liver of both experimental lines of animals after exposure to intermittent normobaric hypoxia. The distance between adjacent nuclei of hepatocytes decreased. Cytochrome oxidase activity in a suspension of mitochondria increased. These morphological changes took place in the liver parenchyma of the both experimental lines of rats. But they were more pronounced in the liver of rats of the Wistar line than in the SHR line. Our data indicated that dosed normobaric hypoxia had a one-way, stimulating effect on the morphofunctional activity of hepatocytes, but the severity of this effect in rats of different lines was not the same. The obtained data can have not only theoretical value, but also be of some practical interest when using intermittent normobaric hypoxia for medical and health purposes in patients with impaired liver function and arterial hypertension.

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