No abstract

The complex of multicellular parasites of sheep in Gorny Altai is mainly represented by intestinal and pulmonary strongilates, moniesia, dikrocelia and parasitic insects, which involves the use of complex parasiticidal agents of a wide spectrum of activity in the system to minimize the volume and frequency of use of drugs. Ivermectin (IVM) has a nematodocidal and entomocidal effect, fenbendazole (FBD) is an effective nematodocide and cestodocide, triclabendazole (TBD) is a recognized trematodocide. To obtain more effective drugs based on these substances, their mechanochemical modification using polyvinylpyrrolidone (PVP) is proposed. The aim of the work is to show the promise of using mechanochemical technology to improve the physicochemical and biological properties of drug substances and to evaluate the effectiveness of compositions of supramolecular complexes ofpolyvinylpyrrolidone with IVM, FBD and TBD in parasitic sheep infestations. Experiments on the study of the parasitocidal activity of compositions for intestinal helminthiases were carried out on a flock of spontaneously invasive sheep in the Shebalinsky district of the Altai Republic. According to the principle of analogues, 6 experimental (10 animals each) and 2 control (20 animals each) groups were formed. The preparations were given to the sheep individually in the form of an aqueous suspension orally. The study demonstrated the high efficiency of solid dispersion (SD) of the composition FBD / IVM / PVP at a dose of FBD - 3.0, IVM – 0.2 mg / kg b / w when administered orally against gastrointestinal strongylates and moniesia (95.8 and 100% ) and a bloodsucker in sheep (92.5%). SD of the composition TBD / IVM / PVP at a dose of TBD - 3.0, IVM - 0.2 showed high efficacy against gastrointestinal strongylates and dicrocelia (96.8 and 100%) and was not effective against moniesia and sheep blood suckers. The initial substances of FBD and TBD showed significantly less activity during parasitic infestations of sheep. The use of the studied SD suggests the possibility of a significant (three-fold) reduction in the dosage of FBD and TBD without loss of parasiticidal activity.

Equine piroplasmosis is a natural tick-borne infection caused by hemoprotozoan parasites of the order Piroplasmida, Babesia caballi and Theileria equi. Animals that recover from piroplasmosis remain persistently infected carriers and can transmit pathogens to vector ticks. Cases of equine piroplasmosis are periodically observed in Siberia, however, no agent of equine piroplasmosis has yet been genetically characterized in Russia. The aim of this work was studying the prevalence of the infectious agents of piroplasmosis in horses from Siberia and genotyping the detected agents. Blood samples from 155 horses were examined for the presence of Babesia and Theileria DNA by nested PCR with the subsequent sequencing of positive samples. DNA of T. equi was found in blood samples from 57.9 %, 38.5 % and 65.0 % of horses from Novosibirsk province, Irkutsk province, and the Republic of Altai, respectively. T. equi DNA was found in the samples from almost all sampling sites included in this study, indicating that most of the studied sites are endemic for equine theileriosis. Surprisingly, DNA of B. caballi was not found in any of the samples examined, even though this agent had previously been detected in many regions in Russia, including Altai. The analysis of the determined 18S rRNA gene sequences demonstrated that T. equi samples belonged to two genetic groups, which differed significantly by the sequences of the variable (V4) region of the gene. All T. equi sequences from group B were identical and corresponded to T. equi sequences found in the blood of horses from China and Korea, while T. equi sequences from group A differed by 1–5 nucleotide substitutions and were identical to the sequences from the blood of horses from India and Brazil or differed from them by single mismatches. Notably, in this study the presence of etiological agent of piroplasmosis in blood samples from horses in Russia was genetically confirmed for the first time.

Background: Parasitic infections are widespread in sheep farms of the Russian Federation, including Siberia. The infection of sheep with helminths and parasitic arthropods with a range of 70% to 100% in different regions, contributes to a decrease in the productivity and quality of products, and even death of animals. This study aimed to formulate drugs with pronounced parasiticidal effects based on ivermectin and albendazole, widely used to treat animal entomoses and helminth infections. Methods: New formulations in the form of solid dispersed compositions were prepared by mechanochemical modification of ivermectin and albendazole using arabinogalactan polysaccharide. The efficacy of preparations on gastrointestinal strongylosis and monieziosis, and melophagosis of sheep was determined by parasitological examination and analysis of feces and urine. Results: The new formulations demonstrated increased solubility and parasiticidal activity due to the formation of inclusion complexes when interact with water. The maximum efficacy values (> 95% efficiency) against intestinal Strongylida and Moniezia expansa, and ectoparasitic Melophagus ovinus were seen in doses lower than the recommended doses of the starting drugs. Conclusion: The increased parasiticidal activity of innovative compositions can be explained by increased water solubility and bioavailability of the preparations, due to formation of inclusion complexes. The results of this study suggests the possibility of a significant reduction in the dosages of composed substances without losing their parasiticidal activity.

The paper demonstrates the prospects of applying Arabinogalactan for mechanochemical modification of physicochemical and biological properties of antihelminthic substance. The study evaluates the parasitocidal effect of ivermectin (IVER) and albendazole (ABZ) compounds with Arabinogalactan (AG) in the form of solid dispersions (RD) in sheep intestinal helminthiasis. The authors tested compositions of new specimens with the active substance (DV) of ivermectin with three batches of arabinogalactan from different manufacturers in the ratio of 1:10 and compositions of IVER+ABZ+AG in the ratio of 1:1:10.  The experiments on parasitocidal activity of compositions in intestinal helminthiasis were carried out on spontaneously invaded sheep in Shebala district of the Republic of Altai, where 16 experimental and 2 control groups of animals were arranged following the principle of analogues. The sheep received the specimens on an individual basis: as a water suspension orally; composition of IVER+AG dosed as 0,1; 0,05 and 0,025 mg per a kilo of animal weight; composition of IVER+ABZ+AG - in calculation of IVER+ABZ - 0,1 and 0,2 mg of water suspension, albendazole 1, 2, 3 and 5 mg per 1 kg of animal weight. The experiment has shown that parasitocidal activity of laboratory samples of mechanical compositions of ivermectin and different in degree of purification of AB lots do not differ significantly. It is supposed that parasitocidal compositions can be used for non-pharmacopoeia AG samples of animals, which will reduce significantly the cost of the specimen. The efficiency of the composition of IVER+AG dosed 0.1 mg per 1 kg of animal weight is significantly higher than that of the initial specimen. Mechanical composition IVER+ABZ+AH dosed 0.2 mg of hemectin and 2 mg of albendazole per a kilo of animal weight is highly effective (100%) against sheep intestinal helminths and it assumes the possibility of significant (five times) decrease of AHZ dose in the composition with AH without loss of helmintocidal activity.