Tríbulus terrestris L., an annual herb belonging to the Zygophyllaceae family and growing in moderate and tropical climates, has a rich chemical composition of biologically active substances and chemical elements.The aim of the work is a phytochemical study of Tribulus terrestris L. growing in different geographical zones.Materials and methods. The objects of study were herb specimens of Tribulus terrestris L. collected in different habitats. The samples of the raw materials were shade-dried. The determination of saponins in the raw materials, was carried out by high performance liquid chromatography with a mass spectrometric detection (HPLC-MS / MS). The study of the qualitative and quantitative composition of the elements was carried out on an X-ray fluorescence spectrometer.Results. The saponins had been studied by HPLC-MS/MS, according to which in all the studied samples, dioscin and protodioscin were found. Their retention times coincided with the retention times of dioscin and protodioscin standards. It has been established that among the macroelements of Tríbulus terrestris L., potassium and calcium are mostly accumulated. They account for about 90% of the total content of the elements in the plant. It has been revealed that the distribution of macroand microelements in the plant, varies significantly depending on their place and growing conditions.Conclusion. The maximum dioscin content was observed in the samples harvested in Moldova, and the minimum – in the samples from the nursery garden of the All-Russian Scientific Research Institute of medicinal and aromatic plants. The largest amount of protodioscin was found out in the samples from the Crimea, and the minimum – in the samples from Moldova. The carried out study of the elements content of Tríbulus terrestris L. showed that the habitats (geographical zones) in which the studied samples of raw materials had been were collected, affect the accumulation of the elements by the plant. Based on the data obtained, biological absorption series have been compiled for the samples from each habitat.
Introduction. Medicinal plant raw materials are one of the most important sources of herbal remedies used both for the prevention and treatment of a number of diseases [1, 2]. Even with the development of modern science and chemistry, medicinal plant raw materials are widely used in both folk and official medicine. The advantage of medicinal plants is their wide range of biological activity, low toxicity and the possibility of long-term use without significant side effects. Human economic activity has a noticeable negative effect on the condition of wild-growing plants: their stocks are decreasing, and some species disappear altogether. Today, given the high level of development of industry and agriculture, the procurement of raw materials for wild medicinal plants is not always possible. Information on the quantitative assessment of the raw material base of wild medicinal plants in the Middle Urals, the content of biologically active substances in medicinal plant raw materials is partially outdated, which determines the need for their systemic resource study and chemical-pharmacognostic study.Aim. Comprehensive assessment of the state of populations of wild medicinal plants in the Middle Urals.Materials and methods. Determination of stocks of raw materials of the studied species of medicinal plants was carried out on specific thickets according to the generally accepted method. The authenticity of the raw materials was established by a macroscopic method when collecting raw material samples. In the course of the study, samples of medicinal plant materials of 5 types were prepared. The determination and assessment of the main indicators of the good quality of medicinal plant raw materials (the content of active and extractive substances, moisture in the mass upon drying, total ash and ash insoluble in a 10% solution of hydrochloric acid) was carried out according to the methods and requirements of the State Pharmacopoeia of the Russian Federation XIV edition. In the raw materials Artemisiae absinthii herba and Leonuri herba, the amount of extractives was determined by the gravimetric method. The quantitative assessment of the content of essential oil in the samples of Origani vulgaris herba and Tanaceti vulgaris flores was carried out by the method of hydrodistillation. To determine the quantitative content of the sum of flavonoids in Hyperici herba, Artemisiae absinthii herba, Leonuri herba and the sum of flavonoids and phenolcarboxylic acids in Tanaceti vulgaris flores, a spectrophotometric method was used.Results and discussion. In the course of resource and phytochemical studies of representatives of the medicinal flora of the Middle Urals, a comprehensive assessment of the state of populations of wild medicinal plants – sources of medicinal plant raw materials (Origani vulgaris herba, Hyperici herba, Tanaceti vulgaris flores, Artemisiae absinthii herba and Leonuri herba) was carried out. The results are included in the electronic inventory of wild medicinal plants of the Middle Urals.Conclusion. The conducted complex of studies will allow updating information about the medicinal flora of the Middle Urals in order to use raw materials for the creation of medicines.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.