The tissues of the head of honey bees which are kept in the areas with a medium and low level of technogenic burden demonstrate the decrease in the contents of iron, zinc, copper, chromium, nickel, lead and cadmium in comparison with the tissues of the head of honey bees grown in areas with a high level of technogenic burden. Due to the activity of saturated fatty acids with the even and odd number of carbon atoms in chain, monounsaturated fatty acids of n-7 and n-9 families and polyunsaturated fatty acids of n-3 and n-6 families, the contents of anionic fatty acids, which are inaccessible for the bee body, decreases in the tissues of the head of honey bees which are kept in the areas with a medium and low level of technogenic burden in comparison with the tissues of the head of honey bees grown in areas with a high level of technogenic burden. 3. Monounsaturated fatty acids of n-7 and n-9 families and polyunsaturated fatty acids of n-3 and n-6 families in the tissues of the head of honey bees lead to increase of the total number of easily accessible non-esterified fatty acids for the honey bees which are kept in the areas with a medium and low level of technogenic burden in comparison with the tissues of the head of honey bees grown in areas with a high level of technogenic burden. Furthermore, the intensity of transformations of the non-esterified form of linolenic acid in its more long-chain and more unsaturated derivatives rises in the head tissues of the former. This fact indicates that the reduction of technogenic impact on the area results in the increase of the activity of desaturase in the tissues of the head of honey bees. The contents of heavy metals, anionic and non-esterified fatty acids in the tissues of the head of honey bees which are kept in the areas with low level of technogenic burden undergoes the most significant changes.
Meat and meat products are a major part of a person's ration. However, due to their high nutritional value, they are a favorable environment for the development of microorganisms and require refrigerated storage. The purpose of this work was to evaluate the storage methods for refrigerated and frozen beef by microbiological and chemical parameters and to suggest criteria for evaluating beef by the content of psychrotrophic microorganisms. It was found out that the storage of beef meat with an initial mesophilic bacterial content of about 4.88 log CFU.cm-2 of surface and psychrotrophic bacteria 3.79 log CFU.cm-2 at temperature 0 °C is only possible for 8 days, further, the microbiological indices exceed the acceptable standards. Investigation of the dynamics of microflora reproduction during the storage of beef in the frozen state at temperature -2 to -3 °C for 20 days established a decrease in 1.3 times the number of mesophilic bacteria in 10 days of storage. At the same time, the number of psychrotrophic microorganisms during this storage time was increased in 4.5 times, and 20 days in 7.9 times and amounted to 5.3 log CFU.cm-2 of surface area. This indicates that the storage of meat in the frozen state inhibits or completely stops the development of mesophilic microorganisms for 20 days. It was found out that storing of beef in the cooled state at a temperature of 0 ±0.5 °C for more than eight days is impractical, as its biochemical indices are worsening and signs of spoilage are appearing. At the same time, storing of beef in the frozen state at a temperature of -2 to -3 °C for 20 days does not cause such significant biochemical changes as in beef stored in the cooled state at a temperature of 0 ±0.5 °C for 16 days. Therefore, we have experimentally substantiated the quantitative indicators of the content of psychrotrophic microorganisms on the surface of beef intended for storage in a cooled or frozen state. The proposed microbiological criteria will improve the safety of beef.
The article presents the results of the research of the new detergent agent “San-active” for meat processing enterprises. It was established that “San-active” in the concentration from 0.3 to 2.0% is moderately alkaline (concentration of hydrogen ions is 11.44–12.7), at a concentration of 2.5% and above, with very alkaline pH ≥ 13.11 units. In the “San-active” detergent, at the concentration from 0.3 to 2.5%, the surface tension is 34.97–28.24 mN/m. The absorbability of the parts of the technological equipment with the solutions of the “San-active” means sharply increases with increasing concentration. At the temperature of solutions of the medium 19.0 ± 1.0 °С the angle of wetting decreases from 69.8 degrees. at a concentration of 0.3% to 50.5 degrees. at a concentration of 2.5% (in 1.4 times). It has been established that “San-active” in 0.5% concentration provides the bactericidal effect on test cultures of conditionally pathogenic bacteria, spore-forming microorganisms and fungi. The “San-active” agent at 0.5% concentration is bactericidal to S. aureus and E. faecalis cells that are in a biofilm in 10 minutes of exposition. For the inactivation of E. coli and P. aeruginosa cells in a biofilm, it is necessary that the “San-active” acts in a concentration not lower than 0.5% and not less than 30 minutes. The agent shows a washing effect on the evaluation of “good” at 0.5% concentration, and 1.0% and above the concentration on the score “excellent”. “San-active” in the concentration from 1.0 to 2.0% shows very weak corrosion activity on stainless steel. The use of “San-active” detergent for the sanitary treatment of equipment surfaces in the intestinal workshop at the concentration of the working solution 1.0–2.0% and the temperature 60 ± 5 °C for 20 minutes provides 99.9–100% efficiency of sanitary treatment.
Synthetic growth stimulants are widely used to get high productivity of animals. These preparations can accumulate in the meat and their residual quantities will adversely affect the health of consumers. The purpose of the work was to monitor the content of zeranol, growth stimulant of ruminants in beef which goes to meat processing enterprises of the Western region of Ukraine and to determine the effect of heat treatment on its quantity. It was found out that 29.8% of beef samples taken at meat processing enterprises contained a stimulant for the growth of ruminant zeranol. It was found that during the storage of beef samples frozen at a temperature of -18 °C with different content of zeranol there is a decrease in its number. The most intense process of destruction of zeranol occurred during the first month of storage, during this period of time the amount of zeranol is reduced by an average of 20%, regardless of the initial content. Within two months of storage of frozen beef, the content of zeranol decreases by 28.2 ±0.17%, and at the end of the sixth month its quantity decreases to 33.2 ±0.58%. It was also found that the dynamics of zeranol reducing in beef samples with large quantities (22.5 μg.kg-1) and small (2.3 μg.kg-1) were the same. It was set up that during 30 min of meat cooking there was a decrease in the content of zeranol 24.7 ±0.23% and 32.0 ±0.35% for 60 min, compared to its content in fresh meat. At the same time, when stored in the frozen state and subsequent cooking, the reduction of zeranol content in meat was 39.3 ±0.3%. Therefore, it is proposed to revise and amendments into the regulatory documents of Ukraine regarding the control and supervision of the presence of hormone (zeranol) residues in meat and meat products in order to prevent their sale and consumption by humans.
Meat foods matter very much in the feed of man and fold considerable part her food ration. In many countries of the world meat is the basic object of food industry. In our state that determines basic legal and organizational principles of providing of quality and safety of meat products, food products made from them for life and health of population and prevention of negative in fluence on an environment in case processing, packing and moving through the custom border of Ukraine. By the most effective method of providing of safety of food products presently the system НАССР, that is base on implementation of requirements of DSTU 4161–2003, sconfessed in the world, that included general principles of functioning of the system, and also requirements of Regulation of European Parliament and Advice №852/2004. In terms of safety and quality cooked sausages (manufacturer PE «Matviychuk A.V.» Zhytomyr region), sausages (manufacturer SPE «Argon» t. Vinnitsa), small sausages (manufacturer SPE «Marshalok», t. Belaya Tserkov Kiev region) meet the requirements laid down DSTU 4436:2005 and hygienic in the production of these types of meat products. Our country has the Law of Ukraine «On basis principles and requirements for safety and quality of food», which spelled out the need to carry out inspections on compliance with hygienic and sanitary requirements in the production of safe and quality of food. The highest protein content was found in cooked (by the standards according to DSTU 4436:2005 – 12%). Fat content, moisture, starch and sodium chloride were well within the norms set of regulations for there meat products. Also safety measure as sodium nitrite content in sausage products did not exceed permissible levels (less than 0,005 %). MAFAnM lowest content was found in cooked sausages and sausages – 1.21·102 ± 28.82 и 2.82·102±42.54 КUO/g. In small sausages MAFAnM content was increased slightly – 1.16·103±29.67 КUO/g. The content of toxic elements in the investigated samples of meat products was within acceptable levels in accordance with DSTU 4436:2005 and radionuclide 137Cs and 90Sr – did not exceed permissible levels set by GN 6.6.1.1–130–2006.
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