Nm23‐H1 and nm23‐H2 are putative metastasis suppressor genes that encode nucleoside diphosphate kinase (NDPK) A and B. NDPKs form oligomers distributed between soluble and particulate fractions of cells and therefore may exert their effects as either soluble or bound proteins. To determine whether metastasis‐related functions of NDPKs are mediated by their catalytic activity in membrane bound or soluble complexes, we have stably transfected highly metastatic human melanoma Line IV Cl 1 cells with wild‐type and catalytically inactive (H118Y) nm23‐H1 and nm23‐H2 genes and assayed their metastatic potential in nude mice. Transfection with wild‐type nm23‐H1 and nm23‐H2 genes and catalytically inactive nm23‐H1 did not significantly (all p > 0.10) alter the metastatic potential of Line IV Cl 1 cells while transfection with catalytically inactive nm23‐H2 significantly (p < 0.01) reduced their metastatic potential. The lack of effect of transfection with wild‐type and catalytically inactive nm23‐H1 suggests that neither soluble nor membrane bound NDPK A affect the metastatic potential of Line IV Cl 1 cells. The metastasis suppressive effect of catalytically inactive NDPK B overexpression suggests that competition with bound complexes containing catalytically active NDPK B inhibits metastasis of Line IV Cl 1 cells. These results imply that bound NDPK B promotes metastasis and suggest that inhibition of its function or of its binding to critical sites may be a useful approach to limit the development of metastases in human melanoma. Int. J. Cancer 88:547–553, 2000. © 2000 Wiley‐Liss, Inc.

The article presents a modification of the colorimetric method for the preliminary establishment of presence of blood in the stainson the material evidences using benzidine test. The proposed modification is accompanied by photometric accounting and computer processing of the results. Performance, objectivity, as well as other features and advantages of this method when used in forensic practice are described in detail.

The article presents the analysis of experience, both of domestic, and of foreign authors in application of the techniques for identification of the blood in the stains on material evidences. The method of definition of IgG by means of ELISA is compared with other modern techniques applied in Russia. Sensitivity, objectivity, actuality and other qualities and advantages of this method are widely displayed when applied in forensic practice.

Проведен анализ опыта как отечественных, так и зарубежных авторов по применению методик установления наличия слюны на вещественных доказательств. Представлены две колориметрические модификации методик, предназначенных для ориентировочного установления наличия слюны в пятнах, следах и участках на вещественных доказательствах. Предложенная нами модификация сопровождается фотометрическим учетом и компьютерной обработкой результатов. Широко раскрываются производительность, объективность, а также другие возможности и преимущества данных методов при использовании их в судебно-медицинской практике.

No abstract