Aim. To determine the formation of bound amino acids in grain of new wheat varieties and its biological value. Methods. Field, physical-chemical, computational, analysis. Results. The differences in amino acid composi- tion of new varieties and lines of wheat were analyzed. It was established that the highest content of essential amino acids was in the grain of the Kulundynka variety (5.18 %) or 2.5 times higher compared to the standard (2.99 %). Their content in the grain of soft wheat, obtained by the hybridization of Triticum aestivum L./Triti- cum spelta L., was 1.4–1.5 times higher compared to the control. The grain of the soft variety Kulundynka had the highest biological value as the score of essential amino acids was not defi cient and the remaining varieties were defi cient in 2–5 amino acids. Only methionine was defi cient in the grain of soft wheat lines (AAS = 64– 74 %). Conclusions. The content of amino acids in soft wheat grain depends considerably on weather condi- tions, selective-genetic origin of the variety and the line. Glutamic acid, proline, and leucine were found to be most abundant. Out of nine samples of soft wheat tested, only the seed of the Kulundynka variety had a non- defi cient amino acid score (91–298 %), and in the Pannonikus variety methionine was limited (49 %). The best balanced content of amino acids is present in the grain of non-spelt lines, obtained by hybridization of Triticum aestivum L. and Triticum spelta L., namely Р 7 and LPP 1314. The grain of these lines has a non-defi cient amino acid score, more methionine (AAS = 64–74 %), and supplies human daily requirement in the best way. The grain has a high index of complex estimation and metabolization coeffi cient for essential amino acids.

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At present, winter durum wheat is grown after its unpaired predecessors. Under such conditions, fertiliser is important. Therefore, the study of the yield formation and quality of winter durum wheat grain depending on the fertiliser system is relevant. The purpose of this study was to investigate the yield formation and quality of winter durum wheat grain under various fertiliser systems. The study was conducted in conditions of a stationary field experiment of the Uman National University of Horticulture, located in the Right-Bank Forest-Steppe of Ukraine during 2020–2021. The experiment was founded in 2011. The following crops are grown in the four-course field crop rotation: winter wheat, corn, spring barley, soybeans. The experiment scheme includes 11 variants of combinations and separate application of mineral fertilisers, including the control option without fertiliser. Grain harvest was performed by direct combining, protein content and gluten content were determined by infrared spectroscopy using Infratek 1241. Statistical data processing was performed using the STATISTICA 10 software. Yield of winter durum wheat grain significantly increased from the fertiliser. However, the effectiveness of their use varied depending on the year of study. Thus, in 2020, it increased by 1.1–1.2 times (3.9-4.1 t hа-1) with long-term use of nitrogen fertilisers alone. Long-term use of complete mineral fertiliser (N150P60K80) significantly affected the grain yield (4.3 t hа-1) compared to variant N150. In 2021, grain yields increased 1.2–1.4 times, depending on the fertiliser system. Notably, the use of N150P60K40 and N150P30K80 in terms of impact on grain yield was at the level of the variant N150P60K80. Paired combinations of fertiliser application were effective at the level of long-term application of N150P30K40. Application of N75P30K40 provided the formation of only 4% lower grain yield compared to N150P30K40. The protein and gluten content was most affected by the nitrogen component of the complete mineral fertiliser. The conducted studies confirm the high reaction of durum wheat to the use of nitrogen fertilisers. The results obtained can be used to predict the productivity of durum winter wheat depending on soil fertility

It has been established that the imbalance of soil processes caused by insufficient intake of organic matter into the soil causes its degradation, decreased fertility, and humus content. There is a problem of ensuring environmental safety. It was also established that the development of new or the improvement of existing structural and technological solutions for tillage is of particular practical interest. Pre-sowing cultivation of the soil is a thorough closure and preservation of moisture through the creation of the upper mulched surface, the destruction of weeds and the creation of favorable conditions for seed germination at a biologically optimal depth of planting. For the steppe zone of the North Caucasus (Krasnodar Territory, Rostov Region and Stavropol Territory), which is the main grain-sowing zone in Russia, research is needed to develop innovative means for pre-sowing treatment that provides the accumulation and preservation of moisture and nutrients, reduces fuel consumption and stable movement of working bodies in soils having different densities. To solve this problem, the hypothesis was put forward at Kuban Agrarian University that in order to ensure moisture conservation and reduce energy consumption, stability of movement of the working bodies of the device in soils with different densities is required, while the stand must deviate under the influence of external resistance in three planes. The research was developed and protected by the patent “Device for presowing tillage”. Studies have been carried out to optimize the energy costs of an innovative tool for presowing tillage using experimental design methods that confirm effectiveness.

Today, spelt wheat grain is used to produce high quality food. Intermediate wheatgrass is a promising crop for prairie restoration. One of the elements of biologization is the influence of growing crops on the microbiota of soil rhizosphere. The microbiota of spelt wheat and intermediate wheatgrass soil rhizosphere remains insufficiently studied. Aim. To study the number of individual groups of microbiota in dynamics in the rhizosphere of cereal crops (spelt wheat, intermediate wheatgrass) depending on the weather conditions and the phase of plants development. Methods. Classical microbiological, statistical methods were used in the work. In particular, the study of the number of microorganisms of different ecological and trophic groups (ammonifying, nitrifying, cellulolytic and nitrogen-fixing) was carried out according to generally accepted methods in soil microbiology. The reliability of the influence of factors was determined by the probability value «р» level which was calculated using STATISTICA 8 program. Results. The amount of ammonifying and cellulolytic microorganisms in the soil rhizosphere of spelt wheat is significantly higher compared to soft wheat. The rhizosphere microbiota amount of the intermediate wheatgrass on the 2–3 year of cultivation was more resistant to adverse environmental factors compared to soft wheat. The soil rhizosphere microbiota did not change a lot depending on the phase of plant development during the vegetation period of cereal crops (spelt wheat, intermediate wheatgrass). Conclusions. The formation of rhizosphere microbiota of spelt wheat and intermediate wheatgrass was first analyzed under the conditions of the Right-Bank forest-steppe of Ukraine. The conducted studies indicate the feasibility of growing and use of spelt wheat in breeding programs to create cultivars of soft wheat with higher activity of rhizosphere microbiota. The number of ammonifying, nitrifying and cellulolytic microorganisms of soil rhizosphere of intermediate wheatgrass was significantly higher compared to soft wheat during all growth stages. The conducted studies confirm the practical application of intermediate wheatgrass to preserve and increase soil fertility. Intermediate wheatgrass can be grown for up to three years in one field, as microbiological activity reaches its maximum development.