No abstract

Nowadays, due to the mass production of plastic products, the question of the negative impact of bisphenol A on the functioning of organs and systems is acute. However, the question of the effect of this xenobiotic on the functional state of the kidneys remains open. Currently, laser irradiation of various powers is intensively used to correct pathologies. However, its biochemical feathers of the impact on biological objects require detailed research. The work is aimed at the study of nephrotoxic effects of bisphenol A under the conditions of irradiation of animals with a diode laser. ВРА was administered per os daily for 3 days at a dose of 50 mg/kg body weight, which corresponded with LOAEL dose. Irradiation was performed for 2 min with a low-diode laser λ = 650 nm (50 mW, 1.5 J/cm2 and 12.5 mW/cm2). The functional state of the kidneys was performed based on the determination of urea and creatinine content in urine and serum. The content of primary and secondary products of the LP was determined in the kidneys. It is shown, that the administration of bisphenol A is accompanied by a decrease in urea in serum and urine, an increase in serum creatinine and a decrease in this metabolite in urine, which may indicate impaired renal and hepatic function due to the damage of this organ by contaminants due to induction lipid oxidation. This is expressed by an increase in the content of conjugated diene and TBA-active compounds in the kidneys. At the same time, under the irradiation of animals, the concentration of urea and creatinine in the serum decreases compared with the BPA group, and in the urine, these indicators increase. Such results may indicate the normalization of kidney function due to the corrective action of the laser. Thus, the introduction of BPA is accompanied by the induction of LPS processes, which leads to renal dysfunction, and irradiation of animals has a corrective effect of nephrotoxic effects of BPA.

One of these novel application areas is LLLT for drug-induced liver injury. LLLI employs visible (generally red) or near-infrared light generated from a laser or light-emitting diode (LED) system. The light is typical of narrow spectral width between 600-1000 nm. Despite significant research efforts around the effects and molecular mechanisms of LLLT in various cells or tissues, the appropriate doses of radiation, energy densities, time, and irradiation conditions as well as the appropriate individual settings in the various laser devices, which will predictably lead to optimal therapeutic effects, have not been clarified yet. In the present study, the effect of low-level laser irradiation on oxidative stress parameters in rats under the conditions of BPA administration was investigated. Toxic liver injury induced by bisphenol A (BPA) administration at 50 mg/kg body wt by gavage for 3 days once a day. Low-level laser irradiation was performed after each administration of xenobiotic. A laser diode (50 mW) with 650 nm continuous wavelength was applied to the skin surface at the anatomical site of the liver. The administration of BPA leads to the evaluated of free radical generation and decrease in SOD, CAT and GPx activities in the liver of bisphenol A-exposed animals. The enhanced processes of the generation of superoxide radicals and nitric oxide and exhausting level of antioxidant enzymes under the conditions of BPA administration leads to the increased oxidative damage of lipid and protein in subcellular fraction. The low-level laser irradiation of BPA-treated animals leads to the increase of enzymatic activity of antioxidant defences system, a decrease of free-radical production and oxidative protein and lipid damage in microsome and cytosol.