Мақалада консорциумға кіретін сүт қышқылы бактериялары мен лактозаны ашытатын ашытқылардың, микробиология және вирусология ҒӨО-ның микроорганизмдер коллекциясында сақтаудағы, сондай-ақ минералды майда сақталған сүт қышқылы бактерияларының тіршілікке қабылеттілігі мен қышқыл түзетін белсенділігі туралы эксперименттік мәліметтер келтірілген. Алынған мәліметтер сүт қышқылы бактерияларының коллекциялық штамдарымен салыстырғанда консорциумға кіретін сүт қышқылы бактериялары мен лактозаны ашытатын ашытқылардың қышқыл түзу қабілетінің жақсы екенін көрсетеді. The article presents experimental data on the viability and acid-forming activity of lactic acid bacteria and lactose-fermenting yeasts included in the consortium, as well as lactic acid bacteria stored in the collection of microorganisms of the NPC of Microbiology and Virology in mineral oil. The data obtained indicate a good acid-forming ability of the strains of lactic acid bacteria and lactose-fermenting yeasts included in the consortium, compared with the collection strains of lactic acid bacteria.
В статье представлены результаты скрининга коллекционных культур дрожжей на антагонистическую активность против возбудителя бактериального ожога Erwinia amylovora. Из 36 исследованных штаммов дрожжей 4 культуры обладали ингибирующей активностью. К ним относятся: Torulopsis kefir var kumis №114 3, Kluyveromyces marxianus19, Torulopsis sphaerica№117, Saccharomyces cerevisiae (vini) 2 комплекс №20. Зоны подавления роста патогена у них составляли 22, 23,5, 22,5 и 26 мм, соответственно. Наибольшая антагонистическая активность была у штамма дрожжей Saccharomyces cerevisiae (vini) 2 комплекс №20. Анализ компонентного состава 4 дрожжевых культур (Torulopsis kefyr var.kumis №114 3, Kluyveromyces marxianus19, Torulopsis sphaerica№117, Saccharomyces cerevisiae (vini) 2 комплекс №20) показал, что у культур Saccharomyces cerevisiae (vini) 2 комплекс №20 и Torulopsis sphaerica№117 выявлено 13 химических соединений, из которых основное соединение - фенилэтиловый спирт. Его процентное содержание составляет 43,1%. У культуры Torulopsis kefyr var.kumis №114 3 обнаружено 12 химических веществ, из которых основное соединение - изоамиловый спирт - 15,7%. А у культуры Kluyveromyces marxianus 19 10 соединений, основное из которых этилацетат – 12,24%. Полученные данные свидетельствуют о том, что исследованные дрожжевые культуры представляют интерес для дальнейшего изучения их как агентов биоконтроля возбудителя бактериального ожога E. аmylovorа. The article presents the results of screening of collection yeast cultures for antagonistic activity against the causative agent of bacterial burn Erwinia amylovora. Of the 36 yeast strains studied, only 4 cultures had inhibitory activity. These are Torulopsis kefir var kumis №114 3, Kluyveromyces marxianus 19, Torulopsis sphaerica№117, Saccharomyces cerevisiae (vini)2 complex №20. Their pathogen growth suppression zones were (22, 23.5, 22.5 and 26 mm), respectively. The greatest antagonistic activity was in the yeast strain Saccharomyces cerevisiae (vini)2 complex №20. The analysis of the component composition of 4 yeast cultures (Torulopsis kefyr var.kumis №114 3, Kluyveromyces marxianus 19, Torulopsis sphaerica №117, Saccharomyces cerevisiae (vini) 2 complex №20) showed that 13 chemical compounds were detected in cultures of Saccharomyces cerevisiae (vini) 2 complex №20 and Torulopsis sphaerica №117, of which the main compound is Phenylethyl alcohol. Their percentage is 43.1%, respectively. The culture of Torulopsis kefyr var.kumis №114 has 12 chemical compounds, of which the main compound is 1-Butanol, 3-methyl - 15.7%.And the Kluyveromyces marxianus culture has 19 10 compounds, the main compound Ethyl Acetate is 12.24%. The data obtained indicate that the studied yeast cultures are of interest for further study of them as agents of biocontrol against the causative agent of bacterial burn E. amylovora.
Изучена способность коллекционных молочнокислых бактерий и молочнокислых бактерий, входящих в консорциум, используемый для получения кисломолочных продуктов, синтезировать экзополисахариды. Установлено, что из 15 испытанных культур четыре (Lactobacillus casei 139, Lactobacillus plantarum №2, Lactobacillus cellоbiosus №20, Lactococcus lactis №K-1) проявляют экзополисахаридную активность. The ability of collection lactic acid bacteria and lactic acid bacteria included in the consortium used to obtain fermented milk products to synthesize exopolysaccharides has been studied. It was found that out of 15 tested cultures, four (Lactobacillus casei 139, Lactobacillus plantarum No. 2, Lactobacillus cellobiosus No. 20, Lactococcus lactis No. K-1) exhibit exopolysaccharide activity.
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