No abstract

An experimental study revealed the effect on modified bioflavonoid on the inhibition of secretion of IFN-γ and IL-2 by ConA-stimulated mononuclear cells. These changes were accompanied by an increase in the secretion of IL-17 and IL-6. Our results suggest the differentiation of CD4(+) T helper cells into Th1 and Th17 subpopulations. The reference drug quercetin dihydrate induced an insignificant change in the level of IL-2 and IL-6 and small increase in IFN-γ content. The content of IL-17 was shown to decrease above the detection limit.

Резюме. В основе патогенеза контактной чувствительности лежит T-клеточный иммунный ответ, вызываемый гаптенами, которые образуют полный антиген, связываясь с белками. Эта реакция проявляется в виде воспаления в месте повторного попадания в организм антигена (обычно в коже). В нашей лаборатории модель контактной чувствительности воспроизводилась на мышах линии СВА, путем накожной сенсибилизации 2,4-динитрофторбензолом. Целью исследования стало выявление механизмов иммуносупрессорного действия флавоноидов корня солодки, используя метод адоптивного переноса контактной чувствительности различными популяциями спленоцитов. Было показано, что флавоноидов корня солодки подавляют адоптивный перенос контактной чувствительности с той или иной силой, в зависимости от популяции клеток с которой взаимодействуют, не вызывая апоптоза клеток-эффекторов.Ключевые слова: флавоноиды, контактная чувствительность, адоптивный перенос. Pavlova S.I., Albegova D.Z., Kyagova A.A., Kozlov I.G. MechanisMs of iMMunosuppressive action of licorice root flavonoids in contact sensitivity in Mice: inhibition of t lyMphocyte effector function Mediated by noneffector cells abstract.Contact sensitivity is a classical T cell-mediated, clinically important phenomenon elicited by small molecular weight molecules (haptens) that bind to host proteins to form a complete antigen. In our model experiments, contact sensitivity was produced in CBA mice, by means of cutaneous sensitization with 2.4-dinitrofluorobenzene. We used an adoptive transfer of contact sensitivity with different populations of splenocytes, in order to detect immunosuppressive effects of licorice (Glycyrrhizae) root flavonoids. Suppression of adoptive transfer of contact sensitivity in mice-recipients to different degree was found, when applying the flavonoids. It was revealed, that this effect depends on the type of target population interacting with flavonoids. We have also demonstrate, that licorice root flavonoids do not induce apoptosis of effector cells.

We studied the effect of modified bioflavonoid and reference drug quercetin dihydrate on proliferation of mononuclears triggered by T- and B-cell mitogens. Lymphocytes were in vivo pretreated with the examined agents followed by their explantation and in vitro activation with T- and B-cell mitogens in cell culture. Intraperitoneal injection of modified bioflavonoid and quercetin dihydrate produced a dose-dependent inhibition of proliferation of the in vitroactivated splenocytes; modified bioflavonoid demonstrated higher antiproliferative activity.