No abstract

Analysis of literature data on the distribution and ecology of the yellow wagtail Motacilla citreola Pallas, 1776 (Passeriformes, Motacillidae, Motacillinae), was performed at the turn of the 19th and 20th cen turies. Its current abundance has been estimated. Distribution patterns of the species at the studied territory within the European part of Russia, including the Middle Volga region, have been revealed.

The Veterinary Service of the Russian Federation takes measures to ensure regular control of livestock health status, to prevent infectious diseases and their introduction into the country; and if such diseases are diagnosed, it takes measures to prevent their spread and contain outbreaks as soon as possible. Success of the taken measures depends on the use of various diagnostic, preventive and therapeutic drugs. In order to produce such medicinal products, biofactories use production and reference strains with stable biological properties, which are stored in national collections of microorganisms. The only keeper of glanders strains is the Laboratory for Collection of Strains of Microorganisms in the FSBSI «FCTRBS-ARRVI», subordinated to the Ministry of Agriculture of the Russian Federation. The following steps were taken due to the official request from FKP Kursk Biofactory – BIOK Company for the transfer of Burkholderia mallei production strain 5584 from the collection of the institution: the strain was passaged in golden hamsters, its viability was determined and biological properties of the culture were studied. The strain was transferred in accordance with the established procedure and in compliance with the biosafety requirements. As the work progressed, Burkholderia mallei strain 5584 culture was isolated and freeze-dried. Before the transfer, biological properties of the freeze-dried Burkholderia mallei strain 5584 were studied for their compliance with the passport data. The obtained results showed that the Laboratory for Collection of Strains of Microorganisms in the FSBSI «FCTRBS-ARRVI» provides optimal conditions to preserve the strain viability and initial biological properties after 5 years of storage. Analysis of the data obtained during the transfer of Burkholderia mallei strain 5584 allowed us to assess the actions taken at all stages of the procedure. It was established that the transfer procedure for the requested glanders production strain complied with the biosafety requirements and regulatory framework regulating the process.

The morphology and topography of atypical lymph nodes of Korean water deer (Hydropotes inermis argyropus) is described for the first time. The aim of this work was to study the structural organization of atypical lymph nodes in water deer. Fresh organ samples were taken from 5 adult water deer. Samples are fixed in formalin and embedded in paraffin wax (Hystomix). Stain serial sections, 4-5 μm thick, are stained with hematoxylin-eosin, Masson’s trichrome stain, Gomori’s silver impregnation stain. Atypical lymph nodes in Korean water deer are located on the trachea along the jugular vein and under the trachea on the internal carotid artery and internal jugular vein. The nodes are localized on the adipose tissue of the pericardium in the thoracic cavity, as well as directly on the aortic arch. In the abdominal cavity, nodes are located along the caudal cava and abdominal aorta, as well as in the connective tissue layers of the pancreas. Organs have a dark red color, oval or round shape, its size - from millet grain to peas. Atypical lymph nodes are heterogeneous organs that differ not only from the usual lymph nodes, but also from each other, both in the type of bringing vessels and some features of the structure. Based on a detailed morphological study, atypical lymph nodes in the abdominal cavity were subdivided into 3 types: 1) hemolymphatic nodes; 2) venous hemal nodes and 3) arterial hemal nodes.

No abstract