Introduction: Staphylococcal infections rank high among healthcare-associated infections. Coagulase-negative staphylococci, especially S. epidermidis and S. haemolyticus, often induce inflammatory processes in newborns. In this regard, it is important to study the persistence of these microorganisms in pediatric hospitals using intraspecific differentiation based on the comparison of spectra of their extracellular proteins. Our objective was to study circulation of S. epidermidis and S. haemolyticus strains in a children’s hospital by electrophoretyping of their extracellular proteins. Materials and methods: We studied 277 strains of S. haemolyticus and 267 strains of S. epidermidis isolated from patients and various objects of the hospital environment by obtaining extracellular proteins of the isolates, analyzing them using polyacrylamide gel electrophoresis, and determining their antibiotic resistance and ability to form biofilms. Results: The analysis of electrophoregrams of extracellular proteins of staphylococci revealed groups of identical strains. Isolates from different patients were combined into 21 S. haemolyticus groups comprising of 69 strains and 13 groups of S. epidermidis comprising of 38 strains. All the cultures were methicillin-resistant, with the exception of one group of two S. haemolyticus strains. More than half of the cultures grouped by spectra of extracellular proteins were multidrug resistant. The absolute majority of S. haemolyticus strains (97.2 %) and three quarters of S. epidermidis strains (76.0 %) were able to form biofilms. The average values of the degree of film formation in S. haemolyticus strains were significantly higher than those in S. epidermidis. Conclusions: Of all the studied cultures of staphylococci, 25 % of strains and 14 % of S. epidermidis strains persisted and were endemic in the children’s hospital, including 88.4 % of hemolytic and 42.1 % of epidermal staphylococcus strains for more than a month, and 21.7 % of S. haemolyticus and 21.1 % of S. epidermidis strains for almost a year. The strains were resistant to methicillin, and the isolates of S. haemolyticus had a high ability to form biofilms.

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У порівняльному аспекті на матеріалі латинської та англійської терміносистем досліджується продуктивність суфіксального способу утворення термінів на позначення інфекційних захворювань, спричинених патогенними мікробами, спірохетами, вірусами, та інвазійн

Object of development: database «Strains of coagulase-negative staphylococcus, persisting in hospitals of different profile».The purpose of the study: inclusion of modern information technologies in the system of epidemiological surveillance and monitoring of infections caused by ecovars of coagulasenegative staphylococcus circulating in Nizhny Novgorod. Methodology: source data was converted into a set of related tables and organized into a hierarchical two-tier database. The database contains information about 1045 strains, circulated in hospitals of different profile of Nizhny Novgorod. To form a sample describing of clinical, epidemiological and phenotypic characteristics of strains requests are made. As a result of the query, a table is formed, where each line corresponds to one strain and contains information about the date, source and location of the recovery, the phenotype of antibiotic and phage resistance, the presence of the mecA gene, as well as sensitivity to different classes of disinfectants and the ability to form biofilm. The database is refillable. It was created using Microsoft Office Access with a standard application package. The database includes 17 queries and 14 tables. Type of implementing computer: IBM PC-compatible PC. View and version of the database management system: Access 2002 and later. Operating system version: Windows 7 and later. The developed database allows searching and processing of epidemiologically significant information about strains of coagulaze-negative staphylococcus.The application of the database is to improve the microbiological monitoring system in epidemiological surveillance of healthcare-associated infections.