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The presence of circulating tumor cells in the blood of patients with triple negative breast cancer (early and locally advanced cancer) before and after preoperative chemotherapy was assessed using expression markers. Before therapy, circulating tumor cells were detected in 5 of 13 (38%) patients with early cancer and in 7 of 17 (41.2%) patients with locally advanced cancer. After therapy, the circulating immune cells were detected in one patient with locally advanced cancer, who had no circulating cells before therapy. The tumor was resistant to chemotherapy and the disease progressed. The detected circulating tumor cells were HER-2-positive, while the primary tumor was HER-2-negative. It was concluded that the circulating immune cells can be a potential marker of the efficiency of therapy and predictors of the disease course, while their phenotype can differ from the phenotype of the primary tumor.

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In response to heat shock five isoforms of hsp70 are accumulated in the myocardium of Wistar rats highly resistant to stress in comparison with only 3 isoforms of hsp70 in August rats with a lower resistance to stress. This suggests that genetic mechanisms which determine the stress resistance of a particular strain are probably related to transcription of hsp70 genes.Key Words: stress; heat shock; myocardium; hsp 70; genetic strains Different organisms from procaryotic to high eucaryotic react to environmental stress factors in different ways. At the same time a common feature of cell response to stress in many cases is quick synthesis of so-called heat shock proteins, hsp [13,15]. The main members of the above family are proteins with a molecular weight around 70 kD (hsp70). Hsp70 are known to participate in the restriction of stress-induced damage by disintegrating abnormal protein-protein interactions [13,15]. For instance, experiments on cell cultures from Drosophila melanogaster [1], Syrian hamster [9,10], and rat liver [6,7] have demonstrated a correlation between increased resistance to stress, namely to heat shock, and hsp70 accumulation. At ties [3,4,8,12]. Thus, the role of heat shock proteins in the formation of stress resistance of the organism remains unclear. One possible approach is the study of peculiarities of accumulation of hsp70 in response to a single stress factor in various animal strains with different resistance to stress.In this context the aim of the present study was, first, to compare quantitatively stress resistance in different animal strains by comparing their resistance to heat shock and, second, to evaluate the differences in the accumulation of hsp70 and their isoforms in response to heat shock in rats with different resistance to stress and to compare the differences in hsp70 accumulation with the differences in stress resistance. Rats of the Wistar and August strains were used. MATERIALS AND METHODSExperiments were carried out on Wistar and August rats weighing 200-250 g. Heat shock reproduced after Currie et al. [2] was used as the stress influence. To this end the rats were first narcotized with Nembutal (50 mg/kg, i.p.) and then exposed to 80~ for 20 rain in a special incuba-