The article discusses the effect of a cross-linked copolymer of potassium and ammonium salts of acrylic acid “Aquasin” on in vitro revitalized grape plants when adapting to non-sterile conditions and growing completion before planting in open ground. The research was carried out on the basis of VNIIViV named after Ya.I. Potapenko on the indigenous Don variety Krasnostop Zolotovsky and the indigenous Crimean variety Kandavasta. The aim of the study was to study the usage effectiveness of super absorbents (hydrogels) for the post vitro stage. As a result, the positive effect of adding the water-retaining agent Aquasin to the substrate on the growth and development of plants was noted. This was especially reflected in such indicators as ripening and the shoots’ diameter. In addition, the possibility of preliminary saturation of the super absorbent with immunostimulants or fertilizers is of interest.

The paper reveals the influence exerted on the development of post vitro grape mother plants and various microzones found on the Ust-Donetsk sand massif. The differences in the microzones were in the fertility level and a number of soil properties. The considerable influence provided by the growing conditions not only on the development of plants but also on the survival rate and life expectancy of mother bushes observed during the use of mother plants for 17 years has been noted. To ensure effective land management, a detailed site study and, depending on the differences identified, the use of precision farming techniques is required before laying out the parent plants. The reason for this is that sandy soils, in general, have a high degree of heterogeneity due to physical and chemical characteristics such as flowability, susceptibility to wind transport, low organic substance content, and cation exchange capacity.