The low-temperature, methane-oxidizing activities and species composition of methanotrophic communities in various tundra bog soils were investigated by radioisotopic and immunofluorescent methods. Methanotrophic bacteria carried out the methane oxidation process through all horizons of seasonally thawed layers down to permafrost. The highest activity of the process has been observed in the water surface layer of overmoistured soils and in water-logged moss covers. Up to 40% of(14)CH4 added was converted into(14)CO2, bacterial biomass, and organic exometabolites. By immunofluoresecent analysis it was demonstrated that the representatives of I+X (Methylomonas, Methylobacter, andMethylococcus) and II (Methylosinus, Methylocystis) methanotrophic groups occurred simultaneously in all samples at 61.6% and 38.4%, respectively. The number of methane-oxidizing bacteria in the ecosystems studied was 0.1-22.9×10(6) cells per gram of soil. Methanotrophic organisms ranged from 1% to 23% of the total bacterial number.

The increase in the number of cases of allergic reactions and celiac disease is an important problem. The solution to this problem is the search and development of relevant and effective ways to eliminate gluten. Specific amino acid sequences glutamine and proline determine the resistance to protease hydrolysis of the structural domains of gluten fractions. The analysis of the literature data showed that an alternative to the gluten-free diet is the use of biotechnological methods for modifying ingredients containing gluten. Such methods include the use of leavens on the base of lactic acid bacteria or enzyme preparations containing peptidases specific to gluten biocatalysis. In addition, the pretreatment of raw materials by extrusion cooking contributes to an increase in the degree of gluten hydrolysis. The effect of the thermoplastic extrusion and various enzyme systems containing proteases, amylolytic, cellulolytic and hemicellulolytic enzymes on the changes in the molecular weights of wheat protein fractions was studied. It was found that extrusion as a factor of protein modification significantly affects the proteolysis of wheat proteins using enzyme systems of different substrate specificity. The most effective hydrolysis was shown by the use of a complex enzyme preparation Amyloprotoorizin. including The effect was also noted after bioconversion of non-extruded wheat. An algorithm for the technology of wheat snacks based on the processes of extrusion and biocatalysis of proteins with specific proteases for the elimination of gluten is devepoped. The practical implementation of the technology will make it possible to obtain ready-to-eat snacks, which will be investigated for the preservation or elimination of antigenic properties during clinical trials.

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The protein, amino acid composition of crops used in the production of bakery products has been thoroughly studied. The protein content amounted in wheat (21%), rye (18,2%), corn (13.1 %). Higher biological value proteins differed rye that 46.7% of the essential amino acids. The range for solubility of proteins of wheat, rye and corn was set. The highest albumin content was noted in the composition of proteins of corn flour (15,5%); globulins -rye flour (24,5%), glutelins -in wheat (38.6 %). Fractional composition of proteins in cereals are represented by fractions in the range from 10 to 97 kDa. Wheat raw materials were marked by a high content of high molecular weight proteins to 97 kDa, as well as glyadenovo and glutaminova fractions in the range from 35 to 44 kDa. It is typical of corn to have the polypeptides of a molecular weight of 50 kDa, and as for rye -to 60 kDa . The processing of grain raw materials complexes of proteases, endo -and ecstasty was carried out. Enzymes were selected by culturing the filamentous fungi Aspergillus niger (enzyme system -1) and Aspergillus oryzae (enzyme system -2). The use of enzymes -2 ( 4 units PS/g) was provided by the accumulation of amine nitrogen in the grain fermentalistic 116,1-130.6 signatures mg%. The formation of free amino acids in slurries of rye amounted to 10% of the total. When using the enzyme system -2 there was a significant decrease in the molecular weight of the polypeptides of 35-40 kDa in all samples of grain crops. In fermentalistic -wheat, rye and corn got a reduced content of allergenic proteins,which ensures the production of the modified products of cereal crops with reduced sensitizing activity.

Разработана технология экструдированных безглютеновых снеков с использованием в качестве частичной замены безглютенового сырья - рисовой муки ферментализатом пшеницы, глютен которой предварительно прогидролизован комплексом протеолитических и амилолитических ферментных препаратов. Иммуноферментным методом анализа подтверждено соответствие содержания глютена в сырье и гидролизате требованиям Технического регламента Таможенного союза 027/12, предъявляемым к безглютеновой продукции. Полученный гидролизат насосом-дозатором подавался в камеру экструдера в количестве до 35 % к массе перерабатываемой сухой смеси на основе рисовой муки. Было изучено влияние дозировки ферментолизата пшеницы на режимные параметры экструзии, изменение структурно-механических, цветовых и органолептических характеристик полученных образцов снеков. Установлено, что с увеличением содержания гидролизата в перерабатываемой смеси возрастает влажность экструдата, отобранного после режущего устройства, в процессе экструдирования снижаются значения момента сдвига, давления, температуры экструзии, а также удельного расхода. Изменение количества вносимого ферментолизата пшеницы значимо изменяло структурно-механические показатели экструдатов. В снеках без гидролизата значение твердости составляло 6,7 Н, а с добавлением ферментолизата отмечалось увеличение до 14,6 Н. Обратная тенденция установлена для показателей коэффициента взрыва и количества микроразломов, значения которых с ростом дозировки гидролизата снижались с 8,6 до 4,1 и с 14,3 до 6,0 соответственно. Отмечено увеличение значений хроматических составляющих цвета снеков при добавлении ферментолизата в экструдируемую смесь, при этом варьирование его дозировкой не оказывало значимого влияния на изменение цветовых характеристик. По результатам дегустации более высокие оценки получили образцы снеков с добавлением 15-20 % гидролизата пшеницы. Whole wheat flour has been hydrolyzed by a complex of proteolytic and amylolytic enzymes in order to eliminate gluten and allergenic peptides. The resulting hydrolysate was tested in gluten-free snack extrusion technology as a substitute for rice flour. The enzyme-linked immunosorbent assay confirmed the compliance of the gluten content in raw materials and hydrolysate with the requirements of the Technical Regulations of the Customs Union 027/12 for gluten-free products. The resulting hydrolysate was fed by a metering pump into the extruder chamber in an amount up to 35 % by weight of the processed dry mixture based on rice flour. The effect of the dosage of wheat hydrolysate on the operating parameters of extrusion, the changes in the structural, color and organoleptic characteristics of the snacks was studied. It has been established that the moisture content of the extrudate taken out after the cutting device increases with an increase in the content of hydrolysate in the processed mixture. At the same time, the values of torque, pressure, extrusion temperature, as well as specific mechanical energy decreased. The content of hydrolysate in the mixture significantly influenced the structural properties of snacks. The hardness value of the snacks without hydrolysate was 6.7 N. It increased to 14.6 N with the addition of hydrolysate up to 35 %. The opposite trend was established for sectional expansion index and the number of fractures, the values of which decreased from 8.6 to 4.1 and from 14.3 to 6.0, respectively. Adding wheat hydrolysate was significant in changing the color characteristics of the extrudates, while the dosage value did not have a significant effect on the color of snacks. According to the results of sensory analysis, samples of the extruded snacks with the addition of 15-20 % wheat hydrolysate received higher scores.