Объекты исследования – молочнокислые бактерии (МКБ), выделенные из биологических образцов, и коллекционные штаммы МКБ. Цель – характеристика штаммов пробиотиков для разработки напитков профилактического назначения. В процессе работыпроведен скрининг пробиотически активных штаммов МКБ, в результате которого отобрано 17 наиболее активных культур, среди которых 13 коллекционных штаммов из Биобанка промышленных микроорганизмов Республиканской коллекции микроорганизмов и 4 лабораторных изолята. Изучен пробиотический потенциал бактерий: продукция молочной кислоты, антагонизм к условно-патогенным микроорганизмам, адгезивные свойства, устойчивость к неблагоприятным условиям желудочно-кишечного тракта (ЖКТ). Идентификация с использованием системы Bruker MALDI-TOF Biotyper позволила отнести изоляты к следующим видам: Lactobacillus casei, Lactobacillus paracasei, Lactobacillus plantarum. В результате проведенных исследований созданы консорциумы стартерных культур из штаммов Lactobacillus paracasei 2А, Lactobacillus casei Y1, Lactobacillus plantarum 1А, Lactobacillus plantarum 17 А, Lactobacillus brevis 4 LB B-RKM 0610 и отобран оптимальный вариант, соответствующий основным технологическим характеристикам с учетом биосовместимости штаммов для включения в состав разрабатываемого напитка профилактического назначения. The objects of study are lactic acid bacteria (LAB) isolated from biological samples and collection strains of LAB Purpose - characterization of probiotic strains for the development of preventive drinks In the course of the work, screening of probiotically active strains was carried out, as a result of which 17 cultures were selected as the most active, among them 13 collection strains from the Biobank of Industrial Microorganisms of the Republican Collection of Microorganisms and 4 laboratory isolates. The probiotic potential was studied, among which, the production of lactic acid, antagonism to opportunistic microorganisms, adhesive properties, resistance to adverse conditions of the gastrointestinal tract. Identification using the Bruker MALDI-TOF Biotyper system allowed isolates to be assigned to the following species: Lactobacillus casei, Lactobacillus paracasei, Lactobacillus plantarum. As a result of the research, consortiums of starter cultures were created from strains of Lactobacillus paracasei2A, Lactobacillus casei Y1, Lactobacillus plantarum 1A, Lactobacillus plantarum 17 A, Lactobacillus brevis 4 LB B-RKM 0610 and the optimal variant was selected that corresponds to the main technological characteristics, taking into account the biocompatibility of strains for inclusion in the composition of the drink for preventive purposes.
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