The purpose of the research is determination of optimal muscle tissue areas for confirmation of diagnosis in the lynx by trichinelloscopy.Materials and methods. The distribution of Trichinella sp. larvae were studied in spontaneously infected lynxes by the trichinelloscopy method when examining 72 sections in a compressorium. In addition to calculating the total number of isolated larvae and the percentage in muscles, we recalculated per 1 g of animal muscles and performed morphometry of the area and perimeter of Trichinella sp. capsules from various muscles, and calculated the ratio of the capsule length and width, and the M value (arithmetic mean): the area of the capsule and its perimeter. We determined the percentage ratio of the larva size to the capsule and the position in the myosymplast using the Vision Bio system (Epi 2014). The results were statistically processed using the Microsoft Excel software package. The larvae were photographed directly from the screen with digital signal processed by the Vision Bio system (Epi 2014).Results and discussion. It has been found that the capsules in the muscles are more often rounded (90% of all Trichinella sp. have a length-to-width ratio of 1.1 : 1). However, there are also oval capsules, the largest number of which was recorded in the masticatory muscles (33% of the total capsules in the masticatory muscles). When studying the selective expansion of larvae, it was found that the masticatory muscles, tongue, diaphragm and gastrocnemius muscles were the most populated. Conclusion. We identified high prevalence and intensity of infection in the lynx, which indicates not only the epizootic significance of the lynx in the spread of trichinellosis in the Kirov Region, but also its epidemiological significance as a source of infection. Due to the fact that the meat of this animal is eaten and there is a risk of human infection, it is necessary to examine the carcasses of these animals for trichinellosis by the compressor trichinelloscopy method or by the peptolysis method.

Trichinellosis is the most dangerous zoonosis of badger. Contamination of Meles meles and Meles leucurus an T. spiralis of Kirov area is investigated. It has been studied badger meat naturally infeсted with T. spiralis of the Кirov region. Trichinella larvae were found in all investigated slides of diafragm. Meles as a source of infection play the leading role in the synantropic infection. Meth-ods of trichinelloscopy can be used for postmortal-diagnostics of trichinellosis is aimed at detection of sources of infection and prevention of helminth zoonosis in human.

The experiment used white mice, which were divided into 4 groups. The first group of mice was injected with a saline solution with 0.04 mg of Trichinella antigen obtained by the Magat method subcutaneously, the second group of mice was injected with azoxymer bromide (polyoxidonium) at a dose of 0.004 mg/mouse, the third group was infected with Trichinella larvae without preliminary administration of drugs, group 4 served as a control. A week after the last injection of drugs, mice were infected with invasive Trichinella larvae at a dose of 40 larvae/animal. Evaluation of the protective properties of polyoxidonium in laboratory models was carried out by determining the intensity of invasion in studies of all muscle groups of the animal by the method of peptolysis and histological material to assess the cellular composition of the spleen. The protective effectiveness of polyoxidonium was 99.43%. The rearrangement of the structure of the spleen under the influence of an immunostimulant indicated a high degree of reactivity of the spleen of animals in response to the introduction of polyoxidonium (the ratio of red and white pulp and their cellular composition changes).

The silver-black fox belonging to the LLC breeding farm “Vyatka” of the Kirov region was used in the work. We used Arkusit, TU 9325-005-51022776-2007, (Developed by the N.M. Emanuel Institute of Biochemphysics and the K.I.Skriabin Moscow State Academy of Medical Sciences), is introduced without any time limits. 2 groups were formed from breeding females of the silver-black fox, according to the principle of analogous groups: control (n = 1235) and experimental (n = 1164). From breeding males of the silver-black fox, 2 groups were formed according to the principle of analogous groups: control (n = 243) and experimental (n = 217). During the experiment, animals of both groups received the general economic ration. Preparation Arkusit was introduced into the feed of breeding animals in 10-day courses with 10-day breaks for 2 months before rut and before whelping at a dose of 0.004 mg / kg of body weight. As a result, there is a decrease in the number of missed and an increase in the number of safely whelped females, an increase in the safety of live puppies and, as a consequence, an increase in the number of registered puppies per successfully whelped female from 4.9 in the control group to 5.6 puppies in the experimental group. Preparation Arcusit promotes an increase in the number of working males, their polygamy, an increase in the number of covered and safely whelped females, an increase in the number of puppies born per male (5.87 puppies) in comparison with the control group.

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