The effect of glycerol solutions of various concentrations and exposure times on the effectiveness of optical clearing (OC) in intact pig skin was studied in the analysis of Raman spectra recorded at different depths. It was found that a solution of 80% glycerol and 20% dimethyl sulfoxide (DMSO) penetrated through the stratum corneum for 45 min. An increase in OC was achieved using mixtures of optical clearing agents (OCA) with DMSO and distilled water. So, the greatest OC efficiency was observed using a mixture of 60% glycerol and 40% water for 45 min (an increase of 3.4 times at a depth of 80 μm). Thus, the possibility of monitoring the optical parameters of the skin at a depth of about 80 μm using the confocal microscopy method of combination scattering.