Transplantaciyu fekal'noj mikrobioty (TFM) naznachayut v kachestve terapii dlya lecheniya razlichnyh patologij zheludochno-kishechnogo trakta. Podbor donora yavlyaetsya odnim iz naibolee vazhnyh i znachimyh etapov dlya TFM. Osoboe vnimanie v poslednee vremya udelyayut probleme nalichiya genov ustojchivosti k raznym gruppam antibiotikov v biomateriale. Cel'yu issledovaniya bylo provesti analiz vstrechaemosti geneticheskih markerov lekarstvennoj ustojchivosti mef i ermB sredi raznyh vozrastnyh grupp naseleniya, vklyuchaya mladencev na grudnom vskarmlivanii, a takzhe opredelit' mikrobiologicheskij sostav distal'noj chasti kishechnika u potencial'no zdorovyh dobrovol'cev donorov TFM. Vsego bylo proanalizirovano 52 obrazca biologicheskogo materala (46 obrazcov kala i shest' — grudnogo moloka) metodom polimeraznoj cepnoj reakcii v rezhime real'nogo vremeni. Gen ustojchivosti k makrolidam (mef) sredi raznyh vozrastnyh grupp byl vyyavlen v 97,8% obrazcah kala, gen ustojchivosti k makrolidam, linkozamidam, streptograminu (ermB) — v 93,5%. V otdel'no vydelennoj gruppe «mat' – ditya» gen mef obnaruzhen vo vsekh obrazcah grudnogo moloka i kala. Gen ermB v etoj gruppe podtverzhden v trekh iz shesti obrazcov grudnogo moloka i chetyrekh iz shesti obrazcov kala mladencev. V rezul'tate detekcii geneticheskih determinat mef i ermB ne tol'ko sredi vzroslogo naseleniya, no i u mladencev, bylo vydvinuto predpolozhenie, chto ispol'zovanie transplantata (kal), soderzhashchego dannye geny dopustimo dlya TFM. Analiz mikrobiologicheskogo sostava kala 23 zdorovyh dobrovol'cev — potencial'nyh donorov TFM, pokazal ochen' nizkij procent sootvetstviya (8,7%) normam mikrobioty distal'noj chasti kishechnika.
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