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Abstract.1 We propose an approach to the preparation of viscoelastic systems based on chitosan without crosslinking agents. It is the formation of an additional network of physical links by introducing a low molecular weight modifier -ethanol or glycerol. It turns out that gelation of chitosan in the solution is accompanied by the formation of additional meshes of physical links, nodes or aggregates of chitosan macromolecules formed in a mixed solvent of acetic acid-water-glycerol or acetic acid-waterethanol. It promotes an earlier formation of viscoelastic properties, which eventually leads to gelation at lower polymer concentrations than in the case of chitosan solutions without alcohol additives. The resulted gelled systems are characterized by good biocompatibility, and therefore can be used as polymeric forms of drugs.

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1 -ГБОУ ВПО "Башкирский государственный медицинский университет" Минздрава России: 450000, Россия, Уфа, ул. Ленина, 3; 2 -ГБОУ ВПО "Башкирский государственный университет", Центр микро и наномасштабной динамики дисперсных систем: 450076, Россия, Уфа, ул. З.Валиди, 32; 3 -ФГБУ "НИИ пульмонологии" ФМБА России: 105077, Москва, ул. 11 я Парковая, 32, корп. 4 РезюмеМетодом атомно силовой микроскопии проведен анализ вязкоупругих свойств (силы адгезии и жесткости мембраны) нейтрофилов, изучены их взаимосвязи с биомаркерами системного воспаления (СВ) у больных хронической обструктивной болезнью легких(ХОБЛ) в зависимости от наличия легочной гипертензии (ЛГ). В режиме силовой спектроскопии определена сила адгезии и количественная оценка жесткости (модуля Юнга) клеточной мембраны нейтрофилов. По результатам исследования показано, что у больных ХОБЛ + ЛГ наблюдается значимое увеличение жесткости мембраны (повышение значения модуля Юнга) и силы адгезии нейтрофилов по срав нению с контролем и больными без ЛГ. Показана тесная взаимосвязь исследуемых показателей нейтрофилов и биомаркеров СВ. Ключевые слова: хроническая обструктивная болезнь легких, легочная гипертензия, атомно силовая микроскопия, нейтрофилы, сила адгезии, жесткость мембраны, биомаркеры воспаления. SummaryThe aim of this study was to investigate blood neutrophil functional properties and the relationships to systemic inflammation in order to better understand a role of systemic inflammation for PH occurrence in COPD patients. Methods. This was a local open comparative study involving 30 patients with COPD (15 patients with PH and 15 patients without PH). The blood neutrophil functional properties (cell membrane stiffness and adhesion force) were measured using the atomic force microscopy. The power spectroscopy was used to quantitatively assess the adhesion force and neutrophil cell membrane stiffness (the Young's modulus). Results. Blood neutrophil and thrombocyte numbers, CRP and fibrinogen levels were sig nificantly higher in patients with COPD + PH compared to COPD patients without PH. Relationships were found between systemic inflammation biomarkers and systolic pulmonary artery pressure. Conclusion. The results demonstrated that the membrane stiffness and the adhesion force were significantly increased in COPD patients with PH compared to controls and to COPD patients without PH. These neutrophil parameters were close ly related to systemic inflammation..

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