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Upcoming professional sports authorities seek rapid noninvasive biosensing tools for regular monitoring of athletes' physiological states. The analysis of saliva through luminescence‐based biosensors has been perceived as a suitable candidate for such purposes. The present study reports a qualitative bioluminescence assay based on a coupled enzyme system that consists of bacterial luciferase (BLuc) and nicotinamide adenine dinucleotide (NADH):flavin mononucleotide (FMN) oxidoreductase (Red), BLuc‐Red, for the express diagnostics of athletes' stress levels before and after physical exertion. The volunteers who participated in the study were grouped as freestyle wrestlers and students who adapted to different levels of physical activities. Under physical exertion modelling conditions, the influence of participant saliva on BLuc‐Red catalyzed light emission was investigated. Results showed a significant increase in residual luminescence (Iexp, mean maximum bioluminescence intensity of the experimental measurement (Iexp); Ic, luminescence intensity in control; Iexp/Ic, %) values for participants in the wrestler group while a decrease in the student group (P < 0.05). Such contrasting residual luminescence values in both groups were found to be dependent on the catalase activity of saliva. The proposed bioluminescence assay can be utilized as a potential nonspecific biosensing tool for determining the physical state of athletes under high loads.

1Сибирский федеральный университет, Красноярск 660041, Российская Федерация 2 Обособленное подразделение Институт биофизики СО РАН, Красноярск 660036, Российская Федерация 3 Научно-исследовательский институт медицинских проблем Севера, Красноярск 660022, Российская Федерация Цель исследования. Выявление влияния слюны спортсменов разной квалификации на интенсивность биолюминесцентного свечения фермента-тивной тест -системы для оценки их физической подготовленности. Материал и методы. Исследована слюна нетренированных студентов и спортсменов разной спортивной квалификации. Функциональ-ное состояние испытуемых оценивали по показателям ФЖЕЛ, ортостатической пробы, ЧСС за минуту в покое и после физической нагруз-ки. Тестирование слюны проводили до и после тренировок биолюминесцентным методом на основе биферментной системы НАДН:ФМ-оксидоредуктаза+люцефераза и методом Н2О2-люминол-зависимой хемилюминесценции. Результаты. Снижение ингибирования интенсивности свечения биолюминесценции и повышение активности антиоксидантной системы слю-ны зависело от уровня тренированности спортсмена. Выявлено снижение величины остаточного свечения после тренировки при низкой фи-зической нагрузке и возрастание -при большой нагрузке на организм. Наибольшие значения изменения остаточного свечения коррелируют с высокими значениями ЧСС. Спортсмены высокой квалификации, отличающиеся высокими показателями функционального состояния, такими как ФЖЕЛ и ЧСС после тренировки, имели наибольшее изменение остаточного свечения в день большой физической нагрузки, что свидетель-ствовало об их хорошей физической подготовленности. Максимальное значение изменения остаточного свечения у спортсменов средней ква-лификации достигалось в середине тренировочного процесса и ниже, чем у высококвалифицированных спортсменов, что характеризовало их физическую подготовленность невысокой. Заключение. Биолюминесцентное ферментативное тестирование слюны спортсменов можно считать перспективным направлением в спортив-ной медицине в качестве тест -контроля в тренировочном процессе с целью его коррекции. Ключевые слова: слюна, биолюминесцентное тестирование, хемилюминесцентный анализ, спортсмены, спортивная квалификация, физическая подготовленность, спортивная медицина. The aim of the research. To identify the influence of athletes saliva of different qualifications on the intensity of bioluminescent glow at the enzymatic test system for assessing their physical preparedness. Material and methods. The saliva of untrained students and athletes of different sports qualifications has been studied. Functional status of the subjects was assessed by FVC, orthostatic test, HR for one minute at rest and after exercise. Saliva testing was performed before and after training by bioluminescent method based on bifermental NADH system: FM-oxidoreductase+luciferase and by H2O2 of luminol-dependent chemiluminescence method.Results. The reduction in inhibition of the luminescence intensity of bioluminescence and the growth the activity of the antioxidant system of saliva depended on the level...

The paper considers the creation of a diagnostic system based on morphological and biophysical indicators that allows us to understand the influence of the climatic component, as well as stress factors on the general physiological state of young people for further correction of preventive measures and preservation of the health of the peoples of the Far North. The aim of the study is a comprehensive assessment of the health of young people living in the Far North based on the results of bioluminescent saliva activity, stress-emotional state obtained before and after intense mental stress, as well as anthropometric and bioimpedance indicators. 117 students of the Medical Institute of Surgut State University took part in the survey at a young age (18-23 years) during the educational process (rest) and during the examination session (mental load) in the first half of the year of study (autumn-winter 2021). The stress-emotional state was assessed using the Spielberger-Khanin test, the functional state of students They were studied using bioluminescent salivary fluid testing, anthropometric and bioimpedance methods. Samples were obtained by spitting unstimulated saliva into a test tube. As a result of the study, regional features of the physique of young people were revealed, which indicates the formation of a phenotype adapted to living conditions in the northern region. The relationship between the stress-emotional status of adolescents and the level of inhibition of the bioluminescent enzymatic system by saliva samples taken before and after mental stress was shown. The value of the residual glow had an increased indicator for the state of rest and significantly reduced the intensity of the bioluminescent glow under mental stress. The methodological development obtained in the future will make it possible to effectively manage the methods and methods of diagnosing young people, taking into account the influence of external factors, climatic zones and conditions.

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