During the research conducted at the All-Russian Research Institute for Starch Products there has been developed a technological mode of using cellulolytic enzymes to reduce the viscosity of grain pulp obtained by grinding naked oat grains soaked in a sodium metabisulphite solution. As the experimental data had been processed, the optimum technological parameters of the process were determined: the consumption of the enzyme preparation Viscoferm was 200 g/t of grain and the dura-tion of fermentation by constant stirring for 2.5 hours at pH 4.6 and temperature 50°C. Under laboratory conditions there has been studied the possibility of starch processing of naked oat grain samples Vyatka, Percheron, 857h05, 766 h05 varieties grown in the Federal Agricultural Research Center of the North-East named N.V. Rudnitsky. Technological assessment based on grain processing in the laboratory using the “plant on the table” method has shown that the yield of coarse-grained starch A in the processing of naked oat using cellulolytic enzymes is 51.4-53.9%, i.e. higher than that of filmy oats, rye Falenskaya 4 and Vyatka 2, wheat and triticale. Low starch content in fiber (7.7-8.7% dry substances DS of fiber) was found in comparison with the results obtained from the processing of filmy oats, Falenskaya and Vyatka 2 rye varieties, wheat and triticale (11.2 - 13.9% DS of fiber). Fiber output by the processing of naked oats is 7.3 - 8.8% DS of grain, by the processing of other types of grain 10.3 - 17.5% DS of grain. The yield of small-grain starch B in the processing of the studied varieties of naked oat is 19.2 - 20.8% DS of grain, that is higher than this value obtained by processing of filmy oats and wheat, but lower than by pro-cessing of rye and triticale. Isolated carbohydrate-protein concentrate, including starch B and proteins, is recommended for use with the extract and fiber as a component for the production of feed.
Background. Innovative technologies for deep processing of grain are widely used in maize grain processing with the release of the grain germ for subsequent extraction of oil and starch from it or production of sugar substitutes in the form of fructose syrup and alcohol, bakery or feed protein. A search for economically valuable sources of maize starch, useful byproducts of its processing, and natural modification of starch for deep processing is vital.Materials and methods. Ten high-starch maize accessions from the VIR collection, isolated by IR spectrometry, were studied. Starch content was measured according to GOST 10845-98, GOST 13586.5-93, GOST 10847-74 and GOST ISO 6647-1-2015 standards. Processing of grain into starch and assessment of the content of amylose and byproducts were carried out at the All-Russian Scientific Research Institute for Starch Products in 2018 according to L. P. Nosovskaya et al. The actual content of starch and its byproducts during deep grain processing was determined. The values of the actual percentage of dry matter (DM, %) in grain, mass fraction of starch, ash content, as well as the yield of extract, pulp, embryo, gluten, process water and starch were determined.Results. Accessions with a high yield of starch (k-4520, k-9301 and k-24730), germ (k-4520. k-8785 and k-24731), pulp (k-4520, k-8785, k-9991 and k-24732) and protein (k8785) were identified as well as those with a percentage of amylopectin in starch above 82% (k-24730 and k-24733) and 100% (k-5461 and k-9991), and amylose above 30% (k4520 and k-9301).Conclusion. Of practical interest in terms of the actual yield of starch (% DM in grain) are accessions k-4520, k-9301, k-24730, k-9991, k-5461 and k-4520. According to the results of breeding tests, accessions k-24730, k-24732 and k-24733 had the following values of starch harvest calculated for grain yield: 4.66, 4.41 and 4.18 t/ha, respectively.
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