Aim. To create a collection of samples from women at different stages of pregnancy to search for early biomarkers of preterm birth.Material and methods. In order to standardize the sample collection, standard operation procedures have been developed with a step-by-step protocol for each research member at the clinical (collection of medical data and biological material) and laboratory (transportation, sample preparation, storage, quality control) stages.Results. As of October 1, 2020, the collection includes peripheral blood samples from 182 women. Whole blood, serum, plasma, buffy coat and urine were collected during pregnancy, and placenta and umbilical cord blood samples — during labor. Clinical and medical history data was obtained about each pregnant woman, which includes data on the woman’s health status, the course and outcome of pregnancy. An electronic catalog has been created with information on samples (data on clinical characteristics and the number of aliquots of each sample type). The quality control (assessment of DNA and microRNA) was carried out, which showed the compliance of the obtained samples with the quality criteria and the preservation of initial characteristics during long-term storage. On the basis of collection, a study has begun to assess the level of microRNA expression in various types of biomaterial, in order to search for early biomarkers of premature birth.Conclusion. The creation of a collection of samples from pregnant women is a significant groundwork for future fundamental and applied research in various fields of biomedicine. This collection may provide an in-depth study of the pathogenesis of various pregnancy complications and the development of new methods for their diagnosis and treatment.-

государственный университет, медицинский факультет, кафедра акушерства и гинекологии Резюме. Наружный генитальный эндометриоз (НГЭ)-заболевание, сопровождающееся формированием очагов гетеротопического эндометрия на брюшине, пролиферацией эндотелиальных клеток и воспалительной реакцией. С целью уточнения динамики воспалительного процесса при НГЭ разной степени тяжести и роли хемокинов и цитокинов в процессах ангиогенеза и воспаления нами исследовалось содержание хемокинов RANTES, IL-8, IP-10, MIG, MCP-1 и цитокинов IL-4, IL-6, IL-10 в перитонеальной жидкости больных НГЭ. Были обследованы 40 женщин репродуктивного возраста с НГЭ, среди них I-II степень тяжести НГЭ были установлены у 20 пациентов, у 20 женщин-III-IV степень тяжести НГЭ. 22 женщины, обратившихся для проведения диагностической лапароскопии в рамках планирования беременности без признаков эндометриоидной болезни, составили группу контроля. Диагноз НГЭ установлен на основании эндоскопических данных и по результатам гистологического исследования. Степень тяжести НГЭ оценивалась по классификации R-AFS. Забор перитонеальной жидкости осуществляли в процессе проведения лапароскопических операций. Концентрацию хемокинов и цитокинов оценивали с помощью метода проточной цитофлуориметрии с использованием тест-систем BD Cytometric Bead Array (BD, США) и проточного цитофлюориметра FACStrack (BD, США). Концентрация RANTES в перитонеальной жидкости была выше при НГЭ I-II степени тяжести по сравнению с НГЭ III-IV степени тяжести и группой контроля. Концентрации IP-10, IL-8, МСР-1, MIG, IL-6 и IL-4 были выше, чем в группе контроля, и коррелировали со степенью тяжести заболевания. В перитонеальной жидкости больных НГЭ нами не обнаружен IL-10. Полученные данные свидетельствуют о несомненном участии указанных цитокинов и хемокинов, обеспечивающих инвазию клеток эндометрия, рост эндометриоидных очагов, формирование микроциркуляторного русла и индукцию процессов воспаления в развитии и прогрессировании НГЭ.

Introduction: Changes in hormonal status in patients with prolonged disorders of consciousness (PDC) remain poorly understood. There are no studies devoted to the investigation of prognostic value of hormonal changes to predict the outcome which is primarily due to a relatively small number of patients, different variants of structural brain disorders in vegetative state (VS) patients, concomitant somatic pathology including typical complications.The objective: to study the correlation between outcomes in VS patients with hormonal status and the level of natriuretic peptide. Subjects and methods. 275 patients in VS were examined for the time period from 2007 to 2017. 152 patients had sequela of traumatic brain injury (TBI) and 123 patients suffered from hypoxic brain damage. All patients matched the international criteria of VS diagnosis. In all patients, blood samples were collected during the first week after admission to ICU to test hormones and natriuretic peptide levels. ACTH, cortisol, TSH, free T3 and T4 , STH, prolactin and natriuretic peptide were tested in the period from 2 to 4 months of staying in VS. In men, the level of total testosterone, LH and FSH was additionally tested. The obtained data were compared with VS outcome.Results. The tested hormones were stably insufficient only in few VS patients. None of the tested hormones of the hypothalamic-pituitary-adrenal axis made a reliable criterion for predicting VS outcome. The tendency of disrupted rhythm of cortisol secretion was found to be most frequent and consistent, with higher rates in the evening hours. The average value of STH was higher in men with sequela of traumatic brain injury who had recovered consciousness versus those who remained in VS. A significant decrease in testosterone levels, regardless of age, was found in the patients with TBI sequela. Mean levels of LH were higher in patients with TBI sequela and hypoxia who remained unconscious versus patients who later restored consciousness. The average level of FSH was higher in patients who had recovered consciousness compared to those who remained in chronic VS. The increased level of natriuretic peptide was observed both in patients who remained in chronic VS and in those who restored consciousness.Conclusions. When investigating levels of certain hormones, no specific endocrine background characterizing this category of patients was found. Abnormal rhythms of some hormones secretion, in particular cortisol, can be considered typical of VS patients especially patients with TBI sequela.

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