The aim of the study was to study the prognostic factors of the effectiveness of plasmon photothermal therapy (PPT) in rats with transplanted liver cancer based on an assessment of the degree of tumor vascularization. Prior to any exposure, rats with transplanted liver cancer RS-1 underwent a doppler ultrasonography to assess the degree of vascularization of the transplanted tumors. Half of the animals with transplanted tumors were removed from the experiment after dopplerography to determine the content of vascular factors in blood serum by immunoassay and immunohistochemical methods in tumor tissue. After triple intravenous administration of gold nanorods (GNRs) coated with polyethylene glycol at a dose of 0.4 mg/ml, the transplanted tumors were irradiated percutaneously with infrared laser radiation at a wavelength of 808 nm and a thermography of local tumor heating was conducted. After 24 hours, the animals were removed from the experiment and samples of tumor tissue were taken for histological examination. It was found that the accumulation of gold in the tumor tissue and the effectiveness of PPT after repeated intravenous administration of GNRs are determined by the presence of a formed vasculature in the tumor.

Исследование включает результаты лечения 167 пациентов с мочекаменной болезнью (МКБ): в 1-ю группу включены 80 больных, которым провели 1 сеанс дистанционной литотрипсии (ДЛТ); 2-ю группу составили 87 больных, которым выполнено 2-4 сеанса ДЛТ. Всем пациентам после ДЛТ проводили дуплексное сканирование почечного кровотока, определяли концентрацию креатинина сыворотки крови и проводили расчет скорости клубочковой фильтрации. Методом ИФА исследовали в динамике содержание липокалина-2 (NGAL) и β2-микроглобулина в моче. Все исследования выполняли на 5-7-е, 10-14-е сутки и через 1-3 месяца после лечения. Показано, что ДЛТ вызывает повреждение почечной паренхимы различной степени выраженности, тяжесть которой возрастает при увеличении числа сеансов. К ранним маркерам острого почечного повреждения у пациентов после ДЛТ следует отнести увеличение экскреции с мочой β2-МГ и NGAL. Динамическое дуплексное сканирование почек после ДЛТ позволяет определить механизмы прогрессирования почечной дисфункции.

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Objective: to identify the role simultaneous use of the Vesical Imaging-Reporting and Data System (VI-RADS) and the assessment of serum levels of several mediators and growth factors for the evaluation of metastatic process and disease stage in patients with bladder cancer.Materials and methods. This retrospective study included 85 patients with histologically verified transitional cell (urothelial) bladder carcinoma (stages рТа—TINOMO. [)T2NOMO, рТ3—4NN0M0, and [)T2—4N1—3M1) and 20 healthy controls. In addition to general clinical examinations, all patients have undergone preoperative diffusion-weighted magnetic resonance imaging of the pelvis with background suppression. We calculated the diffusion coefficient and included it into the VI-RADS protocol. All study participants were also tested for their serum levels of vascular endothelial growth factor (VEGF), interleukin-8 (IL-8), monocyte chemoattractant protein 1 (MSP-1), interferon γ (IFN-γ), transforming growth factor β1 (TGF- β1), granulocyte colony-stimulating factor (G-CSF), and granulocyte-macrophage colonystimulating factor (GM-CSF) using enzyme-linked immunosorbent assay (ELISA).Results and conclusion. We found that the preoperative use of VI-RADS together with the assessment of serum levels of pro-inflammatory mediators, colony-stimulating factors, and growth factors in patients with muscle-invasive bladder cancer provide additional information about the activity of malignant transformation in tumor tissue and tumor spread. Their simultaneous use during the examination of patients with muscle-invasive bladder cancer is a promising diagnostic approach to monitor treatment response.

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