The large number of species which cause parasitic infestations and the wide variety of routes and factors of their transmission give great relevance to systematic veterinary-sanitary research into environmental objects, necessarily paying attention to local natural-climatic conditions, social structure and the activities of the population. This problem is most often studied by medical researchers and ecologists, and the extent of studies on this topic from a veterinary-sanitary point of view is insufficient, which conditioned our choice of the current scientific study. Our goal was determining the level of contamination of urbanized territories with exogenous forms of helminths. The research was conducted during 2010–2018 in the territory of Kharkiv Oblast of Ukraine. During the study, we examined 41 samples of soil, extracted both in rural areas, and in urban conditions, and also 100 samples of feces of animals and birds. During the examination of soil from rural areas in Kharkiv Oblast and soil in Kharkiv and Balakleia, we found that it was contaminated with eggs of helminths of different morphotypes. It was determined that mean level of contamination of soil in rural area equaled 12.5%. At the same time, the level of contamination of soil of river banks was 15% at intensity of 52 ± 5 eggs/kg of soil and 10.0% in meadows at intensity of 54 ± 8 eggs/kg of soil. The level of contamination of the soil of park zones of urbanized territories with exogenous stages of helminths was 5–55% and that of residential zones of cities was 20.0–23.3%. In general, in the samples of soil, eggs of Strongilata, Ascaridata, Trichocephalus and Cestoda were isolated, and in the soil of rural areas also eggs of Trematoda class. It was proved that cattle and small ruminants discharge feces into the environment which have highest number of eggs of Strongylata spp. helminths (285 ± 3 eggs/g of feces). Excrement of infested birds contaminate the environment with exogenous forms of helminths of Trichocephalata (Capillaria spp.) suborder (101 ± 7 eggs/g of feces). In cities, dogs and cats, infested with helminths, discharge into the environment feces with eggs of parasites that belong to Toxocara spp. (75 ± 4 eggs/g) and Dipylidium caninum (6 ± 1 eggs/g). Droppings of infested birds contaminate the environment with exogenous forms of helminthes of Strongylata spp. (57 ± 2 eggs/g), Аscaridia spp. (19 ± 4 eggs/g) and Capillaria spp. (11 ± 2 eggs/g).

Today, one of the important problems in the cultivation and maintenance of agricultural and domestic animals, both in industrial and private farms, is the spread of ecto- and endoparasites, which cause a significant decline in animal productivity, endanger their health and cause economic losses. The study of the level of distribution and conducting of diagnostic studies on parasitoses of animals in farms with different production orientation is an important and integral part of the overall complex of preventive and health-improving measures. The research was carried out during 2007–2017 in livestock enterprises of Kharkiv Oblast. As a part of the research work, 540 samples were collected from livestock farms, of which 180 were from the premises for keeping pigs, 100 from premises for keeping sheep, 120 from premises for keeping cattle, 80 from premises for dogs and 60 – from the surrounding territories of the livestock enterprises. We found that the objects of livestock rearing (pig complex, sheep farm, dairy farm, cynological center) in Kharkiv Oblast have a significant level of sanitary contamination with exogenous forms of helminths (21.7–45.6%) and the soil of the territories (20.0–36.6%). From samples taken from premises for pigs, eggs of four morphotypes were isolated (Ascaris suum – 5.3%, Trichuris suis – 14.6%, Oesophagostomum dentatum – 60.6%, Strongyloides ransomi – 19.4%), from premises for sheep – three morphotypes (Dicrocoelium lanceatum – 3.8%, Trichuris ovis – 29.3%, Strongylata spp. – 46.9%), from premises for cows – three morphotypes (Neoascaris vitulorum – 2.7%, Trichuris skrjabini – 12.9%, Strongylata spp. – 34.5%), premises for dogs – four morphotypes (Toxocara canis – 6.1%, Toxascaris leonina – 5.4%, Trichuris vulpis – 20.6%, Ancylostoma caninum – 17.5%). It should be noted that the places most contaminated with eggs of helminths were manure gutters (100%) and the floor of livestock buildings (50.0–86.7%). It has also been determined that, in the conditions of the cynological center, the Musca domestica was the source of environmental contamination with exogenous forms of Toxocara canis and Trichuris vulpis, and that the Muscina stabulans and Stomoxys calcitrans can be the source of spread of larvae of Ancylostoma caninum and eggs of Trichuris vulpis respectively.

One of the problems in growing and keeping poultry, both in industrial and farm and kitchen garden environments is ectoparasites, which are responsible for lowering the productivity of poultry and causing economic losses. The study of the prevalence and diagnosis of bird ectoparasites on poultry farms with different maintenance technologies is important for the further development of integrated methods for combating them. Our research was conducted during 2006–2017 in 16 regions of Ukraine and the Autonomous Republic of Crimea. In total, 38 poultry farms specializing in egg production with poultry kept in battery cages, 18 poultry houses specializing in egg production with poultry kept on the floor and more than 150 farm and kitchen garden poultry houses for keeping chickens, turkeys, quails, guinea fowl and pigeons were examined. We found that, both with battery cages and when poultry are kept on the floor in poultry farms, the most common ectoparasites are chicken mites (Dermanyssus gallinae), which causes the disease of dermanyssiosis. It was determined that the extensivity of the invasion was, on average, from 56% to 80%. Also, in the chickens and turkeys, mallophages (Menopon gallinae, Menacanthus stramineus), which cause mallophagoses, were detected, and the extensivity of the invasion ranged from 27% to 45%. In farms and kitchen gardens where chickens, guinea fowl and quails were kept, the number of chicken mites in 1 g substrate was more than a thousand live mites, in dovecotes chicken mites were found in 50% to 100% of nests. In chickens and turkeys, besides the chicken mite, bird lice were found. In chickens, extensivity of invasion of M. gallinae, M. stramineus, Lipeurus variabilis, ranged from 15% to 22%, in turkeys, extensivity of invasion of M. gallinae and M. stramineus ranged from 16% to 25%. The scaly leg mite (Knemidocoptes mutans), which causes the disease knemidocoptiasis ("lame leg"), was detected in incubating hens over the age of two years, the extensivity of the invasion ranged from 5% to 7%. It was established that in poultry farms, both in battery cages and when the birds were kept on the floor, the main ectoparasite in chickens, guinea fowl and quail is red chicken mite (D. gallinae), and on poultry farms and kitchen gardens in addition to the chicken mite, three types of bird lice (M. gallinae, M. stramineus, L. variabilis) and K. mutans were found.

The development of new import-substituting highly effective and environmentally friendly complex antiparasitic drugs and their implementation in a scientifically grounded system of measures to combat the causative agents of the main invasive diseases of farm animals and poultry remains an urgent task of veterinary medicine. Based on the results of studies of anthelmintic and antiprotozoal action of agents on exogenous forms of parasites, an experimental form of a complex antiparasitic agent "Tinidafen" was created and investigated. An experimental sample in the form of a powder was created on the basis of two active substances – tinidazole and fenbendazole with the addition of excipients (talc, starch). It has been established that the LD50 of "Tinidafen" for white rats with a single dose is 2564.36±349.13 mg/kg. The therapeutic dose of "Tinidafen" for rabbits is 7.5 g per 10 kg of live body weight. The extensefectevity of "Tinidafen" is 99.5% for helminths and 98.7% for eimeria. After administered orally of the agent, helminth eggs in the feces of infested animals were not registered from day 3, and the release of eimeria oocysts was observed until day 5 of the experiment.

In ostrich farming is difficult to obtain the necessary production and economic results without protection of bird health. We determined the incidence of parasitic ostriches during the period of growing and keeping on specialized eco-zoos. Samples from small and large farms, as well as eco-parks in Kharkiv, Dnepr and Donetsk regions of Ukraine, were examined during 2016- 2020. We performed a parasitological study of 159 samples from three species of ostriches (black African ostrich, Australian common Emu (Dromaius), Rhea). Infestation of ostriches of different ages with two types of nematodes and Eimeria was established. We determoined that the population of ostriches in specialized farms and eco-zoos was infested with nematodes and protozoa with an extensiveness of invasion from 7.7% to 71.4% among adult birds, while young ostriches were infested with Emeria from 20.0% to 44.4%. The largest invasion by helminths and protozoa was observed among black African ostriches in Kharkiv region (41.4%), in Dnepr region it was 33.3%, and in Donetsk it reached 34.1%. Among the livestock of ostriches, there were both mono and mixinvasions. Most often, this was an invasion by Trichostrongylus spp. and Eimeria spp. in African black ostriches, capillaries and Eimeria in Emu. In Kharkiv region, the invasion of Capillaria by Trichostrongylus in African ostriches and Trichostrongylus in Emu were found when they were kept together. Acute and chronic eimeriosis disease can lead to the death of ostriches at the age of 3-4 months.