Two approaches are distinguished in modern ecological monitoring. The first one is physicochemical analysis of environmental objects with respect to maximum allowable concentrations (MACs) of chemical substances, which is performed by standards methods in accordance with state regulations. The second approach (biological monitoring) is based on the methodology of biotesting and bio indication. The task of this work is to create biotests for estimation of Al and other metals toxicity in ground water and to compare these results with physicochemical analysis dates. Risk assessment for heavy metals contaminated groundwater was also performed. Risk assessment was performed accordingly EPA US recommendation and gave results about 90 per 100000 citizens for Al and 402 per 100000 for mixture of different heavy metals. For comparison: risk for earth background radiation for Middle Russia is (Individual dose 1 millisivert per year) consist 5 per 100000 people. It was shown that groundwater consist HCO3− ions (360 mg/l), sometimes Al compounds 0.21–0.65 mg/l (MAC for Al is 0.5 mg/l for Russia). Other groundwater contain Hg – 0.004 mg/l (MAC – 0.0005 mg/l); Cr – 0.072 mg/l (MAC – 0.05 mg/l); As – less than 0.03 mg/l (MAC – 0.05 mg/l). We developed plant biotest for estimation of groundwater quality with barley roots, tradescatia and others. Some biotests parameters correlate with HCO3−, Cl−, SO42− and metal ions content positively, for another biotest this correlation is strongly negative. The quality of groundwater near Obninsk and in Kaluga Region is very different but hasn’t been changed since the year 1998.
In light of the session documents (UNSCEAR, 2015(UNSCEAR, , 2016, presented are the results of health risk assessment in drinking water containing tritium as well as results of field observations and bioassay with algae (Lemna minor and Polyrhiza) from water bodies near radiation-dangerous industrial and scientific objects. The prospects for future studies in these areas should be associated with the assessed human radiation dose from organically bound tritium in organs and tissues of people, animals and plants;the search for plants and animals selectively accumulating tritium and its subsequent bioassay in water. It is necessary to develop the approaches to harmonization of tritium standards in water objects and potable water, in particular. Sanitary and hygiene standards are suggested to develop on the basis of health risk assessment in drinking water with tritium. The upcoming trend for assessing the contribution of organically bound tritium to the total human radiation dose is the local irradiation by tritium incorporated into DNA of cells and tissues and radiosensitive organs. The well-known and new methods of microdosimetry of DNA incorporated radionuclides are necessary to solve this problem.Keywords: natural and technogenic tritium, water biomonitoring, Lemna minor, Spirodela polyrhiza, health risk assessment, drinking water, the interventional level for tritium.
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