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The article contains the data on creating and maintaining the collections in vitro of some ornamental plants – lilac, roses, tree peonies. The analysis of morphogenetic processes during microclonal propagation of lilac cultivars demonstrates well-defined correlation between susceptibility of this culture to mineral mixture of nutrient medium and plant genotype. Rose cultivars from different garden groups keep high growth capacity during 12 subculturing cycles on Murashige Skoog medium (MS) supplemented with 0.5 mg/l BAP. For all culturing tree-peonies cultivars Woody Plant Medium (WPM) with double content of Ca2+ ions and total exception of Cl- ions turns out to be more suitable. The opportunity of 10 lilac cultivars and 4 rose cultivars preservation in slow growth cultures was appreciated. The correlation between long-term cultivation in low temperature and genotype of ornamental plants cultivars was shown. Thus, 18 ºС turns out to be suitable for creation of slow-growth collection of all studied rose cultivars, and 14 ºС – only for 'Nina Weibull'. Maintaining of lilac microshoots at 10 ºС during 5 months practically did not act negatively on their viability.

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