A research study was conducted to find the effect aqueous extract mixture of Zingiberofficinale, Carumcapticum, Withania somnifera, Trigonella foenum graecum, Silybummarianum, Allium sativum and Berberis lyceum on the growth performance of internal organs, lipid profile and hepatotoxicity of broiler chicks. A total of 240 day old chicks were purchased and were reared for 35 days in summer month. The birds were raised on conventional deep litter system, in open shed and all the pens/cages were placed in the same shed to ensure the same environment for all of the groups. Each pen was provided with a feeder and drinker. This aqueous extract mixture of plant was then mixed in drinking water of chicks at the rate of @ 0.0, 5.0, 10.0 and 15.0 ml/L for group A, B, C and D, respectively while group A served as a control. Feed and water were provided on calculated basis. Total numbers of chicks were divided into four groups (A, B, C and D) each having 60 chicks. Each group was further subdivided into three groups (replicates) each having 20 chicks. The data were recorded to explore the effect of infusion based plant mixture on the lipid profile and hepatotoxicity. AST and ALP had significantly (P<0.05) decreasing trend in groups B, C & D. ALT was significantly (P<0.05) lower in Group B. Group D had significantly (P<0.05) lower cholesterol, Triglyceride and LDL and higher HDL values. Serum protein were significantly (P<0.05) higher in group A. Group B @ 5.0 ml/L of Aqueous Extract water to improve liver function. Lipid profile could be well controlled, while using plant mixture extract @ 15.0 ml/L of water. Therefore the use of these medicinal plants is of great concern especially for broiler hepatic-toxicity and lipid profile. Keywords: Broilers, lipid profile, hepatotoxicity, higher density lipoprotein.

The biomass of the California redworm hybridis abiologically valuable feed additive for farm animals, poultryand fish. Worms contain a large amount of protein, lipids,vitamins and trace elements. The chemical composition ofworm biomass depends on the nutrient medium on whichit was grown. Worms are able to accumulate trace elementsfrom the nutrient medium in their body. Using this property,we conducted research on the biomass of worms enrichedwith Cobalt with the prospect of its furthe rusein fishfeeding. It was found that increasing the number and weightof worms depends on the content of Cobalt in the nutrientmedium. The in troduction of 20 mg/kg of Cobalt in to thenutrient medium increased the number and weight of wormsweighing 0.4-0.8 g, respectively, by 38.0% and 40.4%. Anincrease in the number of immature worms by 32.2% wasdetected. By adding 40 mg/kg of Cobalt to the nutrientmedium, the umber and weight of worms increase by 45.9and 51.1%, respectively. The introduction of Cobalt in theamount of 160 mg/kg resulted in a decrease in the numberof worms weighing 0.4-0.8 g compared to the experimentalgroups by 6.5-27.7%. It was found that at a dose of Cobalt160 mg/kg, the number of small worms decreased relativeto the experimental groups by 24.0-50.7%. There was adecrease and weight of young worms relative to control by22.4%.It is established that with increasing cobalt content in thenutrient medium increases its concentration in the biomassof worms. The highest content of Cobalt was found in thedrymatter of the biomass of worms grown on a nutrientmedium to which was added the test metalin the amount of160 mg/kg.The biomass of worms grown on a nutrient medium towhich was added 40 mg/kg of the investigated metal canbeuse das a protein supplement with high cobalt content infish feeding. Key words: vermiculture, wormbiomass, metalaccumulation, nutrient medium, Cobalt, atomicabs orptionspectros copy.

Premise of the study was to validate the growth promoting eff ect of protease on the performance and to explore its digestion enhancer eff ect in broiler chicks. For this purpose 4 commercial diets were divided into two types (low and high density) and were enriched with protease using a completely randomized design with 4 replicates per diet having 10 chicks each having totaled 200 poultry broiler chickens (day-old). Until 14 days, no eff ects were observed on chicks however at day 14; little variations were observed on weight gain, feed intake and FCR (feed conversion ratio) among the enzyme enriched diets. At day 28, prominent response of protease supplementation in low protein was procured. The chicks gained 10.06 and 8.0 % more weight on CFP1 than CFG1 and CFG2, respectively. Similar response in FCR was observed and was found to be 0.20 and 0.15 points better on CFP1 than on CFG1 and CFG2, respectively. However, CFP2 failed to show protease effi cacy declining the weight gain by 23.01 % while the FCR by 0.49 points as compared with CFP1. This suggested that the nature of feed ingredients in the diets is important for obtaining maximum benefi t of protease supplementation. The overall performance indicated signifi cant response to enzyme supplemented diets. Among the low protein diets CP digestibility remained unchanged but they were diff erent in sparing AME (apparent metabolizable energy) for chicks. The CFP2 spared 98.21 kcal/Kg more AME than CFP1. However, this increased AME values did not help to boost the performance and was attributed to the widening ratio between CP and AME. These results demonstrated that the overall growth response of chicks was improved on low protein diet enriched with protease. It showed higher CP digestibility and AME values than good quality diets. However, the inconsistent results observed within the two types of diets revealed that the nature of diets might have infl uenced the effi cacy of protease. Key words: Broilers, digestibility, protease, FCR, Feed intake.