No abstract

The aim is to identify the morphological features of the uterus layers in women at different time intervals of the postmortem period as diagnostic criteria for establishing the postmortem interval. Materials and methods: In the study we used surgical and autopsy material – uterine tissue fragments. All materials were divided into two groups. The 1st group (G 1) included surgical material from women (n=6) who underwent removal of the uterus, or uterus with the appendages due to leiomyoma, uterine prolapse. The 2nd group (G 2) included autopsy material from 42 women with known causes of death and postmortem period (from 24 to 48 hours – 6 cases, from 49 to 72 hours – 7 cases, from 73 to 96 hours – 8 cases, from 97 to 120 hours – 6 cases, from 121 to 144 hours – 8 cases, more than 144 hours – 7 cases). Histological and immunohistochemical study methods were used. Results: A comprehensive morphological study of the women uterus revealed a time-dependent increase of postmortem changes in this organ linked with the increase of postmortem period. In cases of postmortem period duration up to 144 hours, the structural elements of the uterine layers were identified. In cases where the duration of the postmortem period was more than 145 hours, microscopically the uterus was represented by eosinophilic fibrous or dusty masses, the histogenesis of which could not be determined. The processes of autolysis occurred more intensely and faster in the mucous membrane of the uterus, in comparison with the muscular and serous membranes, and in the vessels – in their inner membrane, in comparison with the middle and outer membranes. Autolytic changes in the muscular membrane of the uterus and vascular walls occurred more intensely in muscle fibers compared to connective tissue fibers. Conclusions: The histological and immunohistochemical features of the women uterus at different postmortem periods have a certain forensic medical significance and can be used for establishing the postmortem interval.

To optimize the treatment of diseases which are accompanied by disorders of the psycho-emotional sphere, it is necessary to identify differences and expose the pathogenesis of these changes appearance depending on the primary pathology. During the study, changes in the components of behavior in the elevated plus maze were studied in rats with a developed explosion-induced neurotrauma (EINT), hyper- and hypothyroidism. The experiment conduc­ted on 33 Wistar rats, of which 12 sexually mature rats were divided into control and expe­rimental (with an established EINT) group, 21 young rats were divided into 3 groups – with hyperthyroidism, hypothyroidism and a control group. All animals were kept in standard conditions of the vivarium of the DSMU, the studies were carried out in accordance with the requirements of bioethics. Analysis of the results showed that under the action of various pathological factors, different behavioral profiles are formed, which indicate the development of a trivial state during stress-like reactions after neurotrauma or with an altered hormonal state. The behavioral profile of rats after reproduction of the EINT in comparison with the control group indicates the development of stress-like conditions (statistically significant (p≤0.05) increase in upright stands by 62%, an increase in the number of bowel movements by 50%. Hyperthyroidism is characterized by sympathetic nervous system activation, as evidenced by a significant (p≤0.05) increase in both the number of measures in the open sleeves by 70% and a significant (p≤0.05) increase in the time spent in light sleeves by 51%. In hypothyroidism, there is a suppression of the emotional component of the rats behavior, as evidenced by a significant (p≤0.05) reduction of measures by 63% and time spent in light sleeves by 86%, grooming duration by 71%, hanging from the edges of open sleeves by 80% and the number of bolus defecation by 100%. Keywords: thyroid gland, antigen, staphylococcal toxoid, mast cells, experiment, rats.

No abstract

No abstract