We carried out a comparative analysis of morphological pattern and element composition of regenerated bone tissue forming in the metaphysis defects after substitution of these defects with calcium phosphate substance containing low-molecular non-collagen bone proteins with various affinities to ion exchangers. We have found that regenerated tissue in the defects grew from the edges to the center and its element composition depended on the maturity of newly formed bone tissue. Implantation material containing non-collagen bone proteins with various affinities to ion exchangers induced no significant changes in the content of analyzed elements of bone tissue around the defect. The content of analyzed elements in the areas distant from the defect area did not change during the experiment.

Despite the widespread clinical use of distraction osteogenesis for limb lengthening, the cellular and molecular mechanisms by which this surgical treatment promotes new bone formation in humans are not well understood. The aim of the research was to study the levels of growth factors (GFs) in the serum of patients that were undergoing tibial lengthening with the Ilizarov method of distraction osteogenesis. Those were patients with unilateral congenital discrepancy of the tibia (n = 12), unilateral posttraumatic tibial shortening (n = 7), and healthy patients that underwent cosmetic bilateral tibial lengthening (n = 10). The study established that unlike the congenital group, the posttraumatic group and healthy subjects showed a significantly evident increase in the levels of angiogenic GFs in their serum on day 10 of distraction. In the congenital group, the changes were not significant at this time point. The levels of TGF-α, TGF-β1, and TGF-β2 tended to decrease on day 10 of distraction and on day 30 of the post-distraction period in the cosmetic and posttraumatic groups while they grew in the congenital group. Most dynamic changes in the GFs levels during tibial lengthening were noted in the subjects undergoing cosmetic lengthening, and the least ones were in the congenital group.

Российский научный центр «Восстановительная травматология и ортопедия» им. академика Г. А. Илизарова, Курган Biochemical marKers oF osTeogenesis anD inFlammaTion in The BlooD serum in XenoimPlanTaTion nakoskin a. n., Kovin'ka m. a., Talashova i. a., Tushina n. V., luneva s. n. russian scientific center for restorative Traumatology and orthopaedics named after g. a. ilizarov, Kurgan Изучены биохимические показатели костного ремоделирования в сыворотке крови при ксеноимплантации внеклеточного матрикса в костные дефекты у кроликов. В качестве ксеноимплантов использовали материал, полученный по оригинальной технологии из костной ткани быков, лошадей, кроликов. Также изучалась возможность использования комплексных ксеноматериалов на основе костной ткани и антибиотика. Не обнаружено значимых отличий в реакции различных тканей и органов при ксеноиплантации и аллоимплантации. Установлено, что при ксеноимплантации комплексного материала на основе костной ткани и антибиотика наблюдается выраженная воспалительная реакция на имплантацию. Ключевые слова: ксеноимплантация, щелочная фосфатаза, кислая фосфатаза, С-реактичный белок, белковые фракции, замещение дефектов костей Biochemical indices of bone remodeling in the blood serum were studied in the case of xenoimplantation of extracellular matrix into bone defects in rabbits. The material obtained by the original technology from the bone tissue of bulls, horses, rabbits was used as xenoimplants. The possibility of using complex xenon materials based on bone tissue and antibiotic was also studied. There were no significant differences in the response of various tissues and organs with xenoimplantation and alloimplantation. It was found that with xenoimplantation of a complex material on the basis of bone tissue and an antibiotic, a pronounced inflammatory reaction to implantation is observed.

Федеральное государственное бюджетное учреждение «Российский научный центр "Восстановительная травматология и ортопедия" им. акад. Г.А. Илизарова» Министерства здравоохранения Российской Федерации, г. Курган