Objective. To study microbiological peculiarities of intestinal microflora in children of early age with acute intestinal infections.Material and methods. The scientists carried out the bacteriological examination of 338 children with acute intestinal infections, who were on inpatient and outpatient treatment. The age structure of patients on inpatient treatment from 0 to 6 month – 52 (29,1%), 7–12 month – 57 (31,8%), 1–3 years – 70 (39,1%). The distribution of children on outpatient treatment: from 0 to 6 month – 59 (37,0%), 7–12 month – 35 (22,0%), 1–3 years – 65 (41,0%).Results. It was determined that absence of Escherichia coli (55,7%) and bifidobacterium (81,2%) prevailed in patients on inpatient treatment while in patients on outpatient treatment there was the reduction of these bacteria (67,3% and 36,5% accordingly). Among the representatives of opportunistic microflora, the leading place was occupied by Candida albicans, which was diagnosed in 50% of both inpatient and outpatient children. Only 12,8% cases there were diagnosed with Staphylococcus aureus, Proteus, one patient was diagnosed with Klebsiella, and 5 (9,6%) patients were diagnosed with Salmonellaenteritidis.