Liver injury is a major health problem .Continuous exposure to drugs, viral and bacterial infections and environmental toxins can trigger liver injury and eventually lead to various liver diseases. The present investigation aims to elucidate the possible hepatoprotective effect of black cherry on liver injury induced by administration of acetaminophen (APAP) (which known as paracetamol) to adult male albino rats. Animals were divided into 5 groups, each of 6 rats. The first group was kept as normal control group received (basal diet only). The other four groups fed on basal diet and daily oral dose of paracetamol (400 mg/kg body weight) by gastric tube for one week to induce hepato injury. The hepato-injury rats were classified into control (+ve) group and three treated rat groups which administered selymarin 100 mg/kg/day, 10% dried black cherry and 500mg/kg/day black cherry extract. The treatment period was designed for 28days. Total antioxidant and total plyphenols and flavonoids for black cherry extract were analyzed. Administration of APAP caused liver injury in rats evidenced by significant increase in serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), hepatic malondialdehyde (MDA). In addition, significant decreases in hepatic catalase (CAT) and glutathione (GSH). Total (Phenolic, flavonoids and antioxidant) contents were (4.39 ± 0.33& 6.53 ± 0.46 and 85.94 ± 1.67) in black cherry respectively. Treatment with silymarin , dried black cherry and black cherry extract protect against liver injury as evidenced by significant decreases in liver MDA content, In addition, significant increases in hepatic CAT activity and GSH content. In conclusion: black cherry is rich source for polyphenols, flavonoids and total antioxidant sources so it may be have protective role against liver injury in rats.