Seven fungal strains, namely: Aspergillus terreus, A. clavatus, A. niger, A. humicola, A. sydowii, Paecilomyces variotii and Fusarium oxyspourm were isolated from Stelae dates back to the 19 th Dynasty of the New Kingdom of the reign of King Sity in ancient Egypt, respectively. The isolated fungi were identified by studying their macro and micromorphology. Furthermore, nanogel, nanolime and nonosilver were tested to study their ability to inhibit the fungal growth of the isolated fungi. The potent fungus, a. terreus, which showed best result in nanosilver biosynthesis Aspergillus clavatus (25mm) > Aspergillus niger (24mm) > Aspergillus sydowii (22mm) > Paecilomyces variotii (20mm) and Fusarium oxysporm (20mm) > Aspergillus terreus (14mm) > Aspergillus humicola (12mm), was further identified by the molecular technique (18SrRNA). Nanosilver. On the other hand, nanolime followed nanosilver in its antifungal effect against isolated fungal strains and its activities followed the order: Aspergillus niger (22mm). Different nanomaterials (nanogel, nanolime and nanosilver) were prepared either chemically followed by nanogel exhibited the highest antifungal activities against the isolated fungi whereas nanolime exhibited weak antifungal activity. The effect of archaeological pigments (Malachite, Azurite, Egyptian green and blue) on the isolated fungi as antifungal agents was also investigated. It has been found that Azurite and malachite showed a considerable effect more than the Egyptian green and Egyptian blue. The hydrolysis of animal glue, binding material, was also studied by cultivating the isolated fungi in broth medium containing animal glue as nitrogen source and the proteolytic activity of their culture filtrate has been assayed.