In order to search the new anti-cancer drugs inhibiting with cell division cycle CDC25B phosphatase, designing and synthesizing 6-Methylimidazo[1,2-d][1,2,3,4]thiatriazole-5-carboxylic acid derivatives is meaningful. Methods: The objective compounds were synthesized by cyclization, hydrazinolysis, condensation in the basis of 5-amino-1,2,3,4-triazole and bromine ethyl acetoacetate. Result: The five new kinds of compounds were synthesized. Conclusion: The structures of the compounds were determined by elemental analysis, IR, 1 H NMR and MS, furthermore, it was found that the inhibition rates (% inhibition) of the CDC25B were 85.4 and 82.3 by compounds 3 and 4a with a certain biological activity.