A series of new 3,6-disubstituted triazolothiadiazole derivatives 7a～7y containing benzimidazole and arylsulfonyl moities have been synthesized by o-phenylenediamine and chloroacetic acid as starting materials via multistep reactions. The structures of the intermediates 3, 4, 6 and the target compounds 7 were characterized by 1 H NMR, IR spectra and elemental analysis. All synthesized target compounds were screened for their inhibitory activity against cell division cycle 25B phosphatase (Cdc25B) and protein tyrosine phosphatase 1B (PTP1B). The results show that some of them display significant inhibitory activities against Cdc25B and PTP1B. Among them, compound 7d exhibits the highest inhibitory activity against Cdc25B [IC 50 ＝(7.72±0.73) μg/mL] and 7u exhibits the highest inhibitory activity against PTP1B [IC 50 ＝(3.31±0.57) μg/mL]. It is noteworthy that compounds 7b, 7d, 7l, 7t and 7u show higher inhibitory activities against Cdc25B and PTP1B. They can be considered as potential Cdc25B and PTP1B inhibitors, and have great application prospects in the treatment of cancers and diabetes.

A new fluorescence probe L based on carbazole Schiff-base for Cu 2＋ was synthesized. The recognition ability of probe L to metal ions was investigated by UV-Vis and fluorescence spectra. The experimental results showed that the probe L in CH 3 CN displayed a distinct color change from yellow to colorless upon the addition of Cu 2＋ , which can be directly detected by the naked eye for Cu 2＋ . The fluorescence spectra showed that the probe L had high selective and sensitive recognition toward Cu 2＋ in CH 3 CN, the fluorescence intensity was increased with the increase of Cu 2＋ concentration, and it was not affected by other metal ions. The association constant between the probe L and Cu 2＋ was detected to be 1.38×10 4 L/mol and the corresponding detection limit was calculated to be 2.34×10 -7 mol/L. The detection limit of L for Cu 2＋ was far lower than the maximum allowable level of the WHO limit (20 mol/L) for drinking water. The results indicate that the probe L should be applicable to the research on the environmental applications associated with Cu 2＋ .