Sustained release carrier for the bone morphogenetic protein-2 (BMP-2) is currently a hot subject of research on the bone tissue engineering. In this work, two kinds of heparinized silk fibroin scaffolds were prepared. The binding ability to BMP-2, in vitro sustained release property and the alkaline phosphatase (ALP) activity of the scaffolds were investigated. Results showed that the heparinized silk fibroin scaffolds could bind BMP-2 well and sustained release the BMP-2. The scaffolds with BMP-2 could improve the MG-63 cell growth and cell differentiation with high ALP activity. So the heparinized silk fibroin scaffolds could be an ideal sustained release carrier for BMP-2. Keywords silk fibroin scaffold; BMP-2; alkaline phosphatase; sustained release 骨形态发生蛋白(BMPs)是一种多功能生长因子, 属于转化生长因子-β 超家族的成员 [1]. 其中骨形态发生