This study examined the relationships among levels of knowledge, attitude and preventive behavior regarding tuberculosis in service workers. The survey period was 1 -7 October, 2015 with service workers who work in large scale stores in Jeonnam. The collected data were analyzed using an independent t-test, ANOVA, and Pearson's correlation coefficients using the SPSS WIN 12.0 program. The mean score for knowledge about tuberculosis was 14.05 ± 6.39, the mean score of attitude toward tuberculosis was 29.87 ± 4.89 and the mean score of preventive behavior for tuberculosis was 31.44 ± 4.86. Preventive behavior was found to have significant relationships with gender, marital status, smoking, job satisfaction, and subjective health status. A slightly positive correlation was observed between the knowledge of tuberculosis and the attitudes toward tuberculosis. A positive correlation was noted between the attitude toward tuberculosis and preventive behavior for tuberculosis, while there was a negative correlation between the preventive behavior and age. Therefore, is necessary to consider the related factors for the development and implementation of systematic education programs that can encourage and promote preventive behavior for tuberculosis among service workers.