Many researchers reported that artesian pressure exists in thick soft ground of Busan Nakdong river estuary. Artesian pressure in soft ground could affect rate of consolidation, settlement and drainage capasity of prefabricated vertical drain(PVD). This paper investigated consolidation behaviors of soft ground with partially penetrated PVD subjected to artesian pressure. Laboratory tests with 1-dimensional large column equipment and their numerical analyses were carried out. Test results showed that the consolidation settlement of clay ground with artesian pressure was higher than that without artesian pressure. Due to artesian pressure, the dissipation rate of excess pore water pressure was reduced in soft ground with artesian pressure, especially at bottom part of clay ground. Numerical results were in good agreement with experimental test results.
A microorganism capable of producing high level of poly-3-hydoxybutyrate (PHB) from xylose was isolated from soil. The isolated strain J-65 was identified as Bacillus megaterium based on the morphological, biochemical and molecular biological characteristics. The optimum temperature and pH for the growth of B. megaterium J-65 were 37 o C and 8.0, respectively. The optimum medium composition for the cell growth was 2% xylose, 0.25% (NH4)2SO4, 0.3% Na2HPO4․12H2O, and 0.1% KH2PO4. The optimum condition for PHB accumulation was same to the optimum condition for cell growth. Copolymer of β-hydroxybutyric and β-hydroxyvaleric acid was produced when propionic acid was added to shake flasks containing 20 g/l of xylose. Fermenter culture was carried out to produce the high concentration of PHB. In batch culture, cell mass was 9.82 g/l and PHB content was 35% of dry cell weight. PHB produced by B. megaterium J-65 was identified as homopolymer of 3-hydoxybutyric acid by GC and NMR.
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