Abstract:The Baegdusan Volcano which erupted violently at 1000 AD is still have possibility of eruption. Therefore, it is necessary to monitor regularly the possibility of eruption. However, it is very difficult to install regular monitoring system or to get regularly monitored data due to geopolitic problems. This is why we have to develop regular monitoring technique using satellite. The geoid in the Baegdusan Volcanic area calculated from gravity data obtained from GRACE satellite, decreased from 2002 to 2005. The period of decreasing is well matched with time when magma activities were recognized in the Baegdusan Volcanic area. The decrease in geoid is interpreted to be caused by the decrease of water storage. Considering that the amount of rainfall from 2002 and 2005 is almost constant, the decrease in geoid may be related to the magma activity under the Baegdusan Volcano. The geomagnetic total force in the Baegdusan Volcanic area measured by CHAMP satellite, decreased from 2000 to 2005 and increased after 2005. The period of decrease is well matched with the time with increased activity of magma chamber under the Baegdusan Volcano indicating that the decrease of geomagnetic total force is caused by demagnetization of surrounding rocks due to the increase of temperature of magma chamber. These data
1) The gene encoding Thermus thermophilus HJ6 laccase (Tt-laccase) was cloned, sequenced, and comprised of 1,389 nucleotides encoding a protein (462 amino acids) with a predicted molecular mass of 51,049 Da. The deduced amino acid sequence of Tt-laccase showed 99.7% and 44.3% identities to the Thermus thermophilus HB27 laccase and Synechococcus sp. RS9917 laccase, respectively. Tt-laccase gene was expressed as a fusion protein with six histidine residues in E. coli Rosetta-gami (DE3) cells, and the recombinant protein was purified to homogeneity. UV-Vis spectrum analysis revealed that the enzyme has copper atoms, a type I Cu(II) and a type III binuclear Cu(II). The optimum pH for the oxidation of guaiacol was 5.0 and the optimum temperature was 90℃ The half-life of heat inactivation was about 120 min at 90℃ The enzyme reaction was inhibited by sodium azide, L-cystein, EDTA, dithiothreitol, tropolone, and kojic acid. The enzyme oxidized various known laccase substrates, its lowest K m value being for 4-hydroxyindole, highest kcat value for syringaldazine, and highest k cat /K m for guaiacol.
1) In this study, we carried out the development of high performance photobioreactor, which can be used to develop the biological CO 2 fixation technology as well as the renewable biofuels, the microalgae Botryococcus braunii. When B. Braunii was cultured in Anti-biofouling photobioreator, growth rate of it showed about 3 times higher than that of bubble column photobioreactor at the same conditions. In case of photobioreactor without bead, after 3 days culture time, biofouling occur rapidly in wall of the photobioreactor. However, with bead 5% (V/V), biofouling do not occur all experimental days.
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