The pharmacological efficacy of Protaetia (P.) brevitarsis larvae has been described in the Dongui Bogam.It is believed that the larvae are particularly useful for hepatic disorders. However, natural aversion has made it difficult to consume these larvae as food. Thus, we sought to make an eatable form of the larvae by establishing optimal conditions for larvae preparation. Larvae were selectively bred, sterilized, and a powder of larvae generated by freeze-drying. Afterward, the CellTiter 96 ® AQueous Non-Radioactive Cell Proliferation Assay (MTS) with the RAW 264.7 cell line was used to validate the safety of the powder as a food ingredient. We determined that oak sawdust sterilized by water vapor for 5 minutes could be used for larvae feed, and a feeding for 3～5 days followed by a fasting for 3 days were optimal conditions for larvae preparation. In addition, sterilization of larvae at 115 o C and 0.9 kgf/cm 3 (to avoid contamination of pathogenic bacteria and fungi) was successfully applied in the production of edible powder from P. brevitarsis. The optimized processes established in our experiments can be used in the industrial production of P. brevitarsis as a food ingredient.

This study was conducted to evaluate the effects of the mixture ratio of soil amendments blended with coco peat (Coco) and American soldier fly cast (SFC) on the physicochemical properties on the sand green. pH and EC of soil were significantly related to mixture ratio of SFC, Mix1, Mix2 and Mix3. Capillary porosity, air-capillary porosity and total porosity of root zone mixed SFC, Coco, Mix1, Mix2 and Mix3 were met to the USGA green specification. The mixture ratios of Mix1 and Mix2 in root zone were positively related capillary porosity and total porosity, and air-capillary porosity was negatively related capillary porosity and hydraulic conductivity. Capillary porosity of Mix1, Mix2 and Mix3 blended SFC and Coco was affected by SFC and Coco, and total porosity by Coco. These results showed that soil amendments blended SFC and Coco was developed capillary porosity and hydraulic conductivity on the USGA sand green than these of SFC.

ABSTRACT. Soldier fly casts produced by eco-friendly treatment of food waste with American soldier fly was composting cast mixed food waste compost and soldier fly pupa. This study was conducted to evaluate a possibility of soldier fly casts (SFC) as soil amendment by investigating soil properties and zoysiagrass growth. Amendments were selected coco peat, compost and SFC and their ratio mixed with sand were non-amendment (NA), 7% coco peat(Coco), 7% compost (Comp), 3.5% SFC (1/2SFC) and 7% SFC. In soil properties, SFC was increased CEC than NA and T-N, Av-P 2 O 5 and exchangeable K in soil than Coco. Compared with Coco and Comp, turf quality such as turf color index and chlorophyll index in SFC was higher and root length of zoysiagrass longer. Growth of shoot, runner and root in SFC was increase than that of Coco and Comp. These results indicated that 7% SFC applied as soil amendment was improved growth and quality of zoysiagrass by prompting soil productivity and nutrient availability in soil and root growth.

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