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Васюкова, Н И; Зиновьева, С. В.; Il'inskaia, L I; Perekhod, E. A.; Chalenko, G. I.; Герасимова, Н. Г.; Ильина, А. В.; Варламов, В. П.; Озерецковская, О Л

doi:10.1023/a:1002865029994

Cited by 78 publications

(31 citation statements)

References 15 publications

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“…The elicitor was used at a concentration of 100 µ g/ml, which evokes a systemic immune response without causing necrosis in potato tissues, as shown earlier [16].…”

Section: Methodsmentioning

confidence: 99%

Free and conjugated forms of salicylic acid: Their content and role in the potato

Panina

Vasyukova

Ozeretskovskaya

2005

Appl Biochem Microbiol

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Hydrolysis of conjugated forms of salicylic acid and accumulation of its free form was observed after infection of potato tubers ( Solanum tuberosum L.) with an incompatible race of phytophthora or treatment with an elicitor (chitosan). Infection of tubers with a compatible race of the pathogen or treatment with a suppressor (laminarin) decreased both the degree of hydrolysis of conjugated forms of salicylic acid and the accumulation of its free form.

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“…The elicitor was used at a concentration of 100 µ g/ml, which evokes a systemic immune response without causing necrosis in potato tissues, as shown earlier [16].…”

Section: Methodsmentioning

confidence: 99%

Free and conjugated forms of salicylic acid: Their content and role in the potato

Panina

Vasyukova

Ozeretskovskaya

2005

Appl Biochem Microbiol

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“…Chitosans induce the accumulation of the antifungal phytoalexin pisatin in pea pods and antinutrient proteinase inhibitors in tomato leaves (Walker-Simmons et al 1983).Chitosan reduce stomatal aperture by inducing the evolution of reactive oxygen species from guard cells of tomato and Commelina communis (Lee et al 1999). Chitosan also induces the accumulation of phytoalexins in tissues of host plants, decreasesthe total content and changes the composition of free sterols producing adverse effects on infesters, activated chitinases, beta-glucanases, and lipoxygenases, and stimulates the generation of reactive oxygen species (Vasyukova et al 2001). Barley mildew and its elicitor chitosan promotes closed stomata by stimulating guard-cell S-type anion channels (Koers et al 2011).…”

Section: Oligosaccharidementioning

confidence: 99%

“…PLC is activated by Ca 2+ and IP3 can transportCa 2+ from intracellular stores into the cytoplasm (Aharon et al 1998).Thus, IP3-Ca 2+ signaling pathway is thought to be the main regulatory mechanism of inducing phytoalexin (Altuzar-Molina et al 2011)and cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) are involved in anion channel and Ca 2 + flow, both are important signal channels of accumulation of secondary metabolites in many medical plants (Pietrowska-Borek et al 2014, Wu et al 2008. Fungal elicitor is recognized by the receptorsin the plant, followed with Ca 2+ burst in many medical plants (Du et al 2015, Genre et al 2013, Lecourieux et al 2002.Ca 2+ is a significant second messager involved in the signal transduction progress of endophytic fungi-induced secondary metabolite accumulation in medical plants (Vasyukova et al 2001 to the enhancement of the activity of phospholipase C, which activates the biosynthetic pathways of polyphosphoinositide sugar leading in the improvement of the quantity of phytoalexin.…”

Section: Ion Fluxes and Ca 2+ Signalingmentioning

confidence: 99%

The regulatory mechanism of fungal elicitor-induced secondary metabolite biosynthesis in medical plants

Zhai

Jia

Chen

et al. 2016

Critical Reviews in Microbiology

144

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A wide range of external stress stimuli trigger plant cells to undergo complex network of reactions that ultimately lead to the synthesis and accumulation of secondary metabolites. Accumulation of such metabolites often occurs in plants subjected to stresses including various elicitors or signal molecules. Throughout evolution, endophytic fungi, an important constituent in the environment of medicinal plants have known to form long-term stable and mutually beneficial symbiosis with medicinal plants. The endophytic fungal elicitor can rapidly and specifically induce the expression of specific genes in medicinal plants which can result in the activation of a series of specific secondary metabolic pathways resulting in the significant accumulation of active ingredients. Here we summarize the progress made on the mechanisms of fungal elicitor including elicitor signal recognition, signal transduction, gene expression and activation of the key enzymes and its application to this process. This paper provides guidance on studies which may be conducted to promote the efficient synthesis and accumulation of active ingredients by the endogenous fungal elicitor in medicinal plant cells, and provides new ideas and methods of studying the regulation of secondary metabolism in medicinal plants.

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“…The GPC equipment consisted of two TSK Gel connected columns (TSK G5000-PW and TSK G3000-PW (Tokyo, Japan)), TSP P100 pump (Thermoquest, San Jose, CA) and RI 150 refractive index detector (Thermoquest, San Jose, CA). The eluent was 0.2M CH 3 COOH/0.1M CH 3 COONa. Eluent and chitosan sample solutions were filtered through 0.45 mm Millipore filters.…”

Section: Characterizationmentioning

confidence: 99%

“…3 Low-molecular weight chitosan with an average molecular weight more than 5 kDa prevented the rise of serum cholesterol of rats fed cholesterol-enriched diets for 14 days. 4 And chito-oligomers are now proven to be potent angioinhibitory and antitumor 5,6 agents, as shown by inhibition of angiogenesis and inducing apoptosis as a function of DNA fragmentation.…”

Section: Introductionmentioning

confidence: 99%

Effect of immobilized neutral protease on the preparation and physicochemical properties of low molecular weight chitosan and chito‐oligomers

Jin¹,

Du²,

Liang³

et al. 2006

J of Applied Polymer Sci

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Neutral protease was immobilized on glutaraldehyde-pretreated N-succinyl chitosan hydrogel beads and the biocatalyst obtained was used for the preparation of low molecular weight chitosan and chito-oligomers with molecular weight of 1.9-23.5 kDa from commercial chitosan. Factors affecting the chitinolytic hydrolysis were described. The degradation was monitored by gel permeation chromatography. The structure of degraded chitosan was characterized by Fourier transform infrared, X-ray diffraction and liquid chromatography-mass spectrometry. Immobilized neutral protease showed optimal depolymerization at pH 5.7 and 508C. The degree of deacetylation of the hydrolysates did not change compared to that of the initial chitosan. The decrease of molecular weight led to transformation of crystal structure but the chemical structures of residues were not modified. The degree of polymerization of chito-oligomers was mainly from 3 to 8. The method allows cyclic procedures of immobilized enzyme and N-succinyl chitosan support utilization, and is suitable for a large-scale production of the low molecular weight chitosan and chito-oligomers free of protein admixtures.

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Cited by 78 publications

References 15 publications

Free and conjugated forms of salicylic acid: Their content and role in the potato

Free and conjugated forms of salicylic acid: Their content and role in the potato

The regulatory mechanism of fungal elicitor-induced secondary metabolite biosynthesis in medical plants

Effect of immobilized neutral protease on the preparation and physicochemical properties of low molecular weight chitosan and chito‐oligomers

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