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Domenyuk, Valeriy; Verbitskaya, T. G.; Belousov, A. A.; Sivolap, Yu. M.

doi:10.1023/a:1020600804246

Cited by 7 publications

(2 citation statements)

References 20 publications

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“…Table . ISSR primers used in this study and their specifications (Domenyuk et al, 2002;Galvan et al, 2003 The Matthiola species were located in the same clade, while the other species were placed in a separate clade on the phylogenetic dendrogram. The Sterigmostemum and Chorispora genera, which were selected as out-groups, typically have multicellular glands and are found in various genera of the Brassicaceae family.…”

Section: Resultsmentioning

confidence: 99%

“…PCR amplification using ISSR primers (Domenyuk et al, 2002;Galvan et al, 2003) were carried out by means of an Eppendorf Mastercycler Gradient Thermocycler (USA). Each 25-µL PCR reaction consisted of 2.5 µL of PCR buffer (10 mM TRIS/50 mM KCl buffer, pH 8.0), 3 µL of 25 mM MgCl, 0.5 µL of each primer, 0.5 µL of dNTP mix, 0.4 µL of Taq DNA polymerase (Bioron), 4 µL of template DNA, and 14.1 µL of distilled water.…”

Section: Issr Amplificationsmentioning

confidence: 99%

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Study of phylogenetic relationship of Turkish species ofMatthiola (Brassicaceae) based on ISSR amplification

Dogan¹,

Çelik²,

Ünal³

et al. 2016

Turk J Bot

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IntroductionBrassicaceae, the vascular plant family, consists of 321 genera and 3660 species (Al-Shehbaz, 2012). The cosmopolitan Brassicaceae family is abundant in the northern hemisphere. Currently, there are 49 recognized Brassicaceae tribes into which more than 90% of the genera have been allocated based on molecular analysis (Warwick et al., 2010).A recently published study titled "The Families and Genera of the Vascular Plants" reported that the genus Matthiola is represented by 50 species worldwide, but most of them are found in Eurasia and Africa (Appel and Al-Shehbaz, 2003).Matthiola has 10 taxa in the flora of Europe (Ball, 1964), 6 in the flora of Palestine (Zohary, 1966), 17 in the flora of Iran (Rechinger, 1968), and 15 in the flora of the former USSR (Komarov, 1977). Matthiola has 10 species in Turkey (Mutlu, 2012). The taxa Matthiola ovatifolia (Boiss.) Boiss. and Matthiola trojana have recently been added to the flora of Turkey (Davis, 1988;Dirmenci et al., 2006).Molecular methods have an important role in the identification of phylogenetic relationships within Brassicaceae. Molecular phylogenetic studies have contributed significantly to the generic delimitation on this family (

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Section: Resultsmentioning

confidence: 99%

Section: Issr Amplificationsmentioning

confidence: 99%

Study of phylogenetic relationship of Turkish species ofMatthiola (Brassicaceae) based on ISSR amplification

Dogan¹,

Çelik²,

Ünal³

et al. 2016

Turk J Bot

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Identification of new stock [Matthiola incana (L.) R. Br] cultivars with high fertility through morphological and molecular markers

Ho¹,

Ri²,

Ri³

2022

Genet Resour Crop Evol

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Traditional Management of Maize in the Sierra Sur, Oaxaca, Maintains Moderate Levels of Genetic Diversity and Low Population Differentiation Among Landraces

Aragón-Martínez,

Serrato-Díaz,

Rocha-Munive

et al. 2023

Econ Bot

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Oaxaca is one of the areas of early maize diversification, with 28 to 35 recognized agronomic races. Campesinos (farmers) cultivate at least ten different races in the Los Loxicha region. There is evidence of introgression between them; however, some traditional practices have promoted further differentiation evidenced by the presence of different landraces, clearly recognized by various morphological and phenological characteristics. Therefore, moderate levels of genetic diversity and low levels of population differentiation are expected. Seven communities belonging to two municipalities were selected in 2005. In total, 140 semistructured interviews were administered, inquiring about cultural practices, which included questions about maize seed, ear, and cob management and selection and maize cultivation. Based on interviews and permissions, 30 maize plants from each of 28 fields (milpas) with maize crop belonging to five landraces (Conejo, Hueso, Tablita, Tepecente, and Pinto) were sampled. From the 82 ISSR loci amplified, Hueso and Conejo exhibited the highest and lowest number of loci (70 and 56 loci, respectively); 72% of loci analyzed in the five populations were polymorphic. The Hueso landrace presented the highest percentage of polymorphism (73%) and Conejo the lowest (54%). The expected heterozygosity (HeT) for the five landraces was 0.225 ± 0.032, and the genetic diversity of Nei (HST) was 0.225 ± 0.034. The population differentiation values obtained with GST indicated that Tablita and Hueso were the least differentiated, while the most differentiated were Pinto and Tepecente. STRUCTURE analysis identified two genetically differentiated groups (k = 2), consistent with the length of their agricultural cycle (number of days until flowering). UPGMA analysis showed that milpas belonging to the same landrace and with a specific agricultural cycle length were grouped, regardless of their geographic distribution. These results indicate that traditional management of maize in the Los Loxicha region, particularly as it relates to the selection of ears and seeds and control of the agricultural cycle, maintains a balance between morphological differentiation, moderate levels of genetic diversity and gene flow, and low levels of population differentiation. This has contributed to the high richness and low evenness of agronomic races reported for the mountainous regions of the Sierra Madre del Sur and for Oaxaca.

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Cited by 7 publications

References 20 publications

Study of phylogenetic relationship of Turkish species ofMatthiola (Brassicaceae) based on ISSR amplification

Study of phylogenetic relationship of Turkish species ofMatthiola (Brassicaceae) based on ISSR amplification

Identification of new stock [Matthiola incana (L.) R. Br] cultivars with high fertility through morphological and molecular markers

Traditional Management of Maize in the Sierra Sur, Oaxaca, Maintains Moderate Levels of Genetic Diversity and Low Population Differentiation Among Landraces

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