050 Comparative Analysis of Cultivated Melon Groups (Cucumis melo L.) using Random Amplified Polymorphic DNA and Simple Sequence Repeat Markers

Staub, Jack E.; Fazio, Gennaro; Horejsi, Thomas; Danin‐Poleg, Yael; Reis, Noa; Katzir, Nurit

doi:10.21273/hortsci.35.3.397b

Cited by 33 publications

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“…This reference array represented accessions of differing geographical origin and the cultivar in C. melo L. subsp. melo accessions belonging to diverse botanical groups (Cantalupensis, Conomon, Inodorus, Flexuosus, and Chito) (Tables 1 and 2) (Staub et al 2000;Mliki et al 2001;Nakata et al 2003). The accessions were obtained from seed companies, public germplasm collections, and from the U.S. National Plant Germplasm Collection, U.S. Department of Agriculture (Table 1).…”

Section: Germplasmmentioning

confidence: 99%

“…In this provisionary grouping, subspecies agrestis include, among others, the varieties conomon and momordica, and subspecies melo include the varieties cantalupensis, inodorus, flexuosus, dudaim, and chito. In order to simplify terminology according to groupings in previous work (Staub et al 2000;Mliki et al 2001) and to allow for comparative analysis of reference accessions from these studies, we designate these varieties herein as botanical groups (i.e., Group Cantalupensis, Inodorus, etc. ).…”

Section: Introductionmentioning

confidence: 99%

“…Some commercial melon types belonging to Group Flexuosus are also cultivated in small areas in Spain for regional domestic markets. The horticultural features of Spanish melon landraces and inbred germplasm (i.e., Casaba) grown for domestic markets differ markedly from other European cultivars (Staub et al 2000). The culinary attributes (mainly taste) of these Spanish melon types appear to have been a reason for their historical retention as unique Casaba cultivars (Esquinas-Alcµzar 1977).…”

Section: Introductionmentioning

confidence: 99%

“…Likewise, a rigorous molecular and morphological appraisal of regional collections could be used to define accessions by geographic regions and provide historical baseline data for future genetic studies (e.g., genetic erosion). Therefore, a study was designed to assess the genetic diversity of the Spanish landrace melon collection housed at the Experimental Station 'La Mayora', Spain, and compare this variation with that of other melon diversity analyses of worldwide scope (Staub et al 2000;Mliki et al 2001). This collection is representative of other regional Spanish collections that have their origins from the comprehensive botanical surveys conducted between 1984 and 1985 (Nuez et al , 1988.…”

Section: Introductionmentioning

confidence: 99%

“…Landraces of this collection are regionally diverse and comprise several Spanish cultivars. The variation detected among Spanish melons in this study was compared to standard reference accessions (Africa, Japan, Crete, U.S., and Europe) (Staub et al 1997(Staub et al , 2000Mliki et al 2001;Nakata et al 2003) using a uniform set of RAPD markers (Staub et al 2000).…”

Section: Introductionmentioning

confidence: 99%

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Genetic analysis of Spanish melon (Cucumis melo L.) germplasm using a standardized molecular-marker array and geographically diverse reference accessions

2003

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Genetic relationships among 125 Spanish melon ( Cucumis melo L.) accessions from a Spanish germplasm collection were assessed using a standard molecular-marker array consisting of 34 random amplified polymorphic DNA (RAPD) markers bands (19 primers) and 72 reference accessions drawn from previous studies. The reference accession array consisted of a broad range [Japanese (19) Crete (17), African (15), and USA and Europe (US/EU, 21)] of horticultural groupings (Group Cantalupensis, Group Conomon, Group Inodorus, Group Flexuosus, and Group Chito), and of melon market classes (e.g., Charentais, U.S. Western and European Shipper types, Ogen, and Galia, Honeydew, and Casaba). Spanish melon accessions (largely Casaba, Group Inodorus) were genetically distinct from the reference accessions and other Group Inodorus melons of different origins. Most African accessions showed common genetic affinities, and grouped with the Group Chito and the Group Conomon accessions examined. Those accession groupings were distinct from all other accessions belonging to Group Cantalupensis, Flexuosus, and Inodorus accessions originating from Crete, Japan, Europe, and the U.S. Genetic diversity was highest in accessions of African origin and lowest in accessions of Spanish origin. Additional RAPD markers (49 primers, 141 bands) and 22 selected agronomic traits (quantitative and qualitative) were then used to assess the genetic diversity among Spanish accessions. While cluster analysis using fruit characteristics grouped accessions into cultivars, RAPD-based genetic-distance estimate did not provide consistent accession groupings either by cultivar or geographic origin. While the highest level of polymorphism was detected among melons originating from the central region of Spain, and in the Rochet cultivar, accessions from the Andalucía region and Green cultivars were comparatively less diverse. These results indicate that the Spanish melon accessions could be used to broaden the genetic base of local and foreign Casaba germplasm, to enhance the genetic diversity of U.S and European commercial melon germplasm, and to delineate collection strategies for acquisition of additional Spanish landraces.

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Section: Germplasmmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Genetic analysis of Spanish melon (Cucumis melo L.) germplasm using a standardized molecular-marker array and geographically diverse reference accessions

2003

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Assessment of genetic relationships in Cucurbita pepo (Cucurbitaceae) using DNA markers

et al. 2002

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Cucurbita pepo (pumpkin, squash, gourd), an economically important species of the Cucurbitaceae, is extremely variable in fruit characteristics. The objective of the present study was to clarify genetic relationships across a broad spectrum of the C. pepo gene pool, with emphasis on domesticates, using AFLP, ISSR and SSR markers. Forty-five accessions were compared for presence or absence of 448 AFLP, 147 ISSR, and 20 SSR bands, their genetic distances (GDs) were estimated and UPGMA cluster analysis was conducted. The results obtained from these three marker systems were highly correlated (P << 0.001). Clustering was in accordance with the division of C. pepo into three subspecies, fraterna, texana and pepo, with the first two less distant to one another than to the last one. Within the clusters, sub-clustering occurred in accordance with fruit shape and size. The subsp. texana cluster consisted of six sub-clusters, one each for the representatives of its five cultivar-groups (Acorn, Crookneck, Scallop, Straightneck and Ovifera Gourd) and wild gourds. Within the subsp. pepo cluster, the representatives of two cultivar-groups (Zucchini and Orange Gourd) formed distinct sub-clusters and the representatives of two other groups (Cocozelle and Vegetable Marrow) tended to sub-cluster separately from one another but formed an assemblage with the representatives of the remaining group (Pumpkin). Within-group GDs were less than corresponding between-group GDs in nearly all comparisons. The smallest-fruited accession, 'Miniature Ball', appears to occupy a genetically central position within C. pepo.

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Comparative study of the discriminating capacity of RAPD, AFLP and SSR markers and of their effectiveness in establishing genetic relationships in olive

et al. 2003

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RAPDs, AFLPs and SSRs were compared in terms of their informativeness and efficiency in a study of genetic diversity and relationships among 32 olive cultivars cultivated in Italy and Spain. SSRs presented a higher level of polymorphism and a greater information content, as assessed by the expected heterozygosity, than AFLPs and RAPDs. The lowest values of expected heterozygosity were obtained for AFLPs, which, nevertheless were the most efficient marker system due to their capacity to reveal the highest number of bands per reaction and because of the high values achieved for a considerable number of indexes. All three techniques discriminated the genotypes very effectively, but only SSRs were able to discriminate the cultivars Frantoio and Cellina. The correlation coefficients of similarity were statistically significant for all three marker systems used but were lower for the SSR data than for RAPDs and AFLPs. For all markers a high similarity in dendrogram topologies was obtained although some differences were observed. All the dendrograms, including that obtained by the combined use of all the marker data, reflect some relationships for most of the cultivars according to their geographic diffusion. AMOVA analysis detected greater genetic differentiation among cultivars within each country than it did between the two countries.

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050 Comparative Analysis of Cultivated Melon Groups (Cucumis melo L.) using Random Amplified Polymorphic DNA and Simple Sequence Repeat Markers

Cited by 33 publications

References 0 publications

Genetic analysis of Spanish melon (Cucumis melo L.) germplasm using a standardized molecular-marker array and geographically diverse reference accessions

Genetic analysis of Spanish melon (Cucumis melo L.) germplasm using a standardized molecular-marker array and geographically diverse reference accessions

Assessment of genetic relationships in Cucurbita pepo (Cucurbitaceae) using DNA markers

Comparative study of the discriminating capacity of RAPD, AFLP and SSR markers and of their effectiveness in establishing genetic relationships in olive

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