1,25(OH)2 vitamin D3 signalling on immature rat Sertoli cells: gamma-glutamyl transpeptidase and glucose metabolism

Abstract

1α,25-Dihydroxyvitamin D 3 (1,25-D 3 ) is critical for the maintenance of normal male reproduction since reduced fertility is observed in vitamin D-deficient rats. Gamma-glutamyl transpeptidase (GGT) is a membranebound enzyme that is localized on Sertoli cells and catalyses the transfer of the gamma-glutamyl residues to an amino acid or peptide acceptor. Sertoli cells are also responsible for providing nutrients, as lactate, to the development of germ cells. The aim of this study was to investigate the effect and the mechanism of action of 1,25-D 3 on GGT on Sertoli cell functions from 30-day-old immature rat testis. Results demonstrated that 1,25-D 3 stimulates GGT activity at Sertoli cells plasma membrane through a PKA-dependent mechanism of action, which was not dependent of active de novo protein synthesis. The hormone increases glucose uptake, as well as lactate production and release by Sertoli cells without altering the reactive oxygen species (ROS) generation. In addition, 1,25-D 3 did not change reduced glutathione (GSH) amount or oxygen consumption, and diminished Sertoli cell death. These findings demonstrate that 1,25-D 3 stimulatory effect on GGT activity, glucose uptake, LDH activity and lactate production seem to be an important contribution of Sertoli cells for germ cells nutrition and for a full and active ongoing spermatogenesis.

A Brazilian pulp and paper mill effluent disrupts energy metabolism in immature rat testis and alters Sertoli cell secretion and mitochondrial activity

“…Studies that show the analysis of the mitochondrial metabolism of Sertoli cells through sensitive methods are scarce in the literature. With the exception of an earlier study by our group (Gonçalves et al, 2017), no other studies on the analysis of the mitochondrial oxidative metabolism of Sertoli cells through a high-resolution respirometry (OROBOROS) method, as used in the present study, were found. Our data show that Sertoli cells incubated 1 h with 4% effluent showed increased oxygen consumption at the maximum capacity of the ETS state, where they are challenged with sequential titration of FCCP decoupling.…”

“…We also detected a significant increased effect of effluent in the ROX state. The residual activity in this state is usually represented by partial reduction of oxygen into reactive oxygen species (Gonçalves et al, 2017). In this case, effluent significantly increased the formation of ROS in 10-day-old rat Sertoli cells.…”

Impact of vitamin D deficiency on mouse sperm structure and function

“…This is an important finding as alterations in paternal chromatin can have dramatic consequences on reproduction especially if the oocyte is unable to repair such paternal nuclear damage. Given the extensive antioxidant and regulatory roles of vitamin D on gene expression, 60‐67 further studies are needed to determine which processes (either in the testis during spermatogenesis or in the epididymis during post‐testicular maturation) could be affected.…”

Ascorbic acid promotes the reproductive function of porcine immature Sertoli cells through transcriptome reprogramming

“…7 C). Since LDH is the key enzyme to catalyze the synthesis of lactate from pyruvate under anaerobic condition [ 10 , 30 ], we also checked the activity of LDH in the present study. And the results showed that AA2P (250 μM, 36 h) treatment could increase the activity of LDH significantly (455.05 vs. 424.67; P < 0.01) ( Fig.…”

“…Later, SCs are found to be able to secret a variety of molecules, such as growth factors, androgen binding protein (ABP), anti-müllerian hormone (AMH), inhibin B (INHB) and lactate, to provide growth signals and nutrients for spermatogenic cells [ [5] , [6] , [7] ]. Furthermore, multiple exogenous factors are discovered to affect the proliferation, apoptosis and function of SCs, including heat stress [ 8 ], metal [ 7 , 9 ], chemicals [ 5 ] and nutrients [ 10 ], and disturb the subsequent development of germ cells [ 7 ].…”